This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Robinson, A.
Right arrow Articles by Tetreault, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Robinson, A.
Right arrow Articles by Tetreault, J.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, Feb 1995, 364-370, Vol 33, No. 2
Copyright © 1995 by the American Society for Microbiology. All rights reserved.

Comparison of Crystal Enteric/Nonfermenter system, API 20E system, and Vitek AutoMicrobic system for identification of gram-negative bacilli

A Robinson, YS McCarter and J Tetreault
Department of Pathology and Laboratory Medicine, Hartford Hospital, Connecticut 06102-5037.

A comparative evaluation of the Crystal Enteric/Nonfermenter system (Crystal; Becton Dickinson, Cockeysville, Md.), API 20E (API; bioMerieux Vitek, Inc., Hazelwood, Mo.), and the Vitek GNI card (Vitek; bioMerieux Vitek) was performed with 512 clinical isolates of gram- negative bacilli, including 381 members of the family Enterobacteriaceae and 131 nonenteric bacilli. With supplemental testing, API, Crystal, and Vitek correctly identified to the genus and species level 505 (98.6%), 489 (95.5%), and 494 (96.5%) of the 512 isolates, respectively. Supplemental testing, as specified by the manufacturer, was required to identify 119 (23.2%), 18 (3.5%), and 5 (1.0%) of the isolates with the three systems, respectively. Of the 381 isolates from the family Enterobacteriaceae, API and Crystal correctly identified 90.3 and 91.6% by 18 to 24 h without supplemental testing, respectively, and Vitek identified 92.4 and 96.1% following 10 and 18 h of incubation, respectively. Of the 131 nonenteric organisms, API and Crystal correctly identified 28.2 and 93.9% by 18 to 24 h without supplemental testing, respectively, and Vitek identified 84.0% by 10 h and 93.9% by 18 h. Errors in identification with each system were infrequent and appeared to be randomly distributed among the genera evaluated. The three systems were comparable in accuracy when either a weighted clinical laboratory profile of organisms or a group of selected isolates in a stress test sample was evaluated (P > 0.05). There were no significant differences between the three systems in their ability to identify either the isolates in the weighted group or those in the stress test (P > 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)


This article has been cited by other articles:

  • Nucera, D. M., Maddox, C. W., Hoien-Dalen, P., Weigel, R. M. (2006). Comparison of API 20E and invA PCR for Identification of Salmonella enterica Isolates from Swine Production Units.. J. Clin. Microbiol. 44: 3388-3390 [Abstract] [Full Text]  
  • O'Hara, C. M., Sowers, E. G., Bopp, C. A., Duda, S. B., Strockbine, N. A. (2003). Accuracy of Six Commercially Available Systems for Identification of Members of the Family Vibrionaceae. J. Clin. Microbiol. 41: 5654-5659 [Abstract] [Full Text]  
  • Leclercq, A., Lambert, B., Pierard, D., Mahillon, J. (2001). Particular Biochemical Profiles for Enterohemorrhagic Escherichia coli O157:H7 Isolates on the ID 32E System. J. Clin. Microbiol. 39: 1161-1164 [Abstract] [Full Text]  
  • O'Hara, C. M., Brenner, F. W., Miller, J. M. (2000). Classification, Identification, and Clinical Significance of Proteus, Providencia, and Morganella. Clin. Microbiol. Rev. 13: 534-546 [Abstract] [Full Text]  
  • Woo, P C Y, Leung, P K L, Leung, K W, Yuen, K Y (2000). Identification by 16S ribosomal RNA gene sequencing of an Enterobacteriaceae species from a bone marrow transplant recipient. Mol. Pathol. 53: 211-215 [Abstract] [Full Text]  
  • Sung, L. L., Yang, D. I., Hung, C. C., Ho, H. T. (2000). Evaluation of autoSCAN-W/A and the Vitek GNI+ AutoMicrobic System for Identification of Non-Glucose-Fermenting Gram-Negative Bacilli. J. Clin. Microbiol. 38: 1127-1130 [Abstract] [Full Text]  
  • Odumeru, J. A., Steele, M., Fruhner, L., Larkin, C., Jiang, J., Mann, E., McNab, W. B. (1999). Evaluation of Accuracy and Repeatability of Identification of Food-Borne Pathogens by Automated Bacterial Identification Systems. J. Clin. Microbiol. 37: 944-949 [Abstract] [Full Text]  
  • Bourbeau, P. P., Heiter, B. J. (1998). Comparison of Vitek GNI and GNI+ Cards for Identification of Gram-Negative Bacteria. J. Clin. Microbiol. 36: 2775-2777 [Abstract] [Full Text]  
  • Jalaluddin, S., Devaster, J.-M., Scheen, R., Gerard, M., Butzler, J.-P. (1998). Molecular Epidemiological Study of Nosocomial Enterobacter aerogenes Isolates in a Belgian Hospital. J. Clin. Microbiol. 36: 1846-1852 [Abstract] [Full Text]  
  • Funke, G., Monnet, D., deBernardis, C., von Graevenitz, A., Freney, J. (1998). Evaluation of the VITEK 2 System for Rapid Identification of Medically Relevant Gram-Negative Rods. J. Clin. Microbiol. 36: 1948-1952 [Abstract] [Full Text]  
  • Denton, M., Kerr, K. G. (1998). Microbiological and Clinical Aspects of Infection Associated with Stenotrophomonas maltophilia. Clin. Microbiol. Rev. 11: 57-80 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»