This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by van der Hoek, L. Articles by Sol, C. J. Search for Related Content PubMed PubMed Citation Articles by van der Hoek, L. Articles by Sol, C. J.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, 03 1995, 581-588, Vol 33, No. 3
Copyright © 1995 by the American Society for Microbiology. All rights reserved.

Isolation of human immunodeficiency virus type 1 (HIV-1) RNA from feces by a simple method and difference between HIV-1 subpopulations in feces and serum

L van der Hoek, R Boom, J Goudsmit, F Snijders and CJ Sol
Human Retrovirus Laboratory, University of Amsterdam, The Netherlands.

A simple method for the isolation and subsequent detection of human immunodeficiency virus type 1 (HIV-1) RNA from feces is described. Viral RNA was isolated by the method developed by Boom et al. (R. Boom, C.J.A. Sol, M.M.M. Salimans, C.L. Jansen, P.M.E. Wertheim-van Dillen, and J. van der Noordaa, J. Clin. Microbiol. 28:495-503, 1990), which was adapted for feces. HIV-1 RNA was detected by reverse transcription (RT) followed by a nested PCR encompassing the V3 region. Reconstruction experiments revealed that the efficiencies of the extraction technique and the subsequent RT-PCR were not considerably affected by the varied composition of feces. The method was applied on fecal specimens from 18 HIV-1-infected individuals, among which were samples that had been stored for 9 years. It appeared that HIV-1 RNA was detectable in the feces of 12 persons (67%). Viral RNA was present in the feces of persons who fulfilled the criteria for CDC class II and CDC class III HIV infection as well as in patients who were diagnosed with AIDS (CDC class IV). Direct sequencing of amplimers obtained from paired fecal and serum specimens showed that differences in sequence heterogeneity existed. In one patient a remarkable difference in the HIV-1 sequences between isolates from feces and serum was observed. In conclusion, HIV-1 RNA is frequently present in the feces of HIV-1- infected individuals, and in some cases the HIV-1 subpopulation in feces differs from the HIV-1 subpopulation in serum.

This article has been cited by other articles:

• Notermans, D. W., Peek, R., de Jong, M. D., Wentink-Bonnema, E. M., Boom, R., van Gool, T. (2005). Detection and Identification of Enterocytozoon bieneusi and Encephalitozoon Species in Stool and Urine Specimens by PCR and Differential Hybridization. J. Clin. Microbiol. 43: 610-614 [Abstract] [Full Text]  
• Ling, B., Santiago, M. L., Meleth, S., Gormus, B., McClure, H. M., Apetrei, C., Hahn, B. H., Marx, P. A. (2003). Noninvasive Detection of New Simian Immunodeficiency Virus Lineages in Captive Sooty Mangabeys: Ability To Amplify Virion RNA from Fecal Samples Correlates with Viral Load in Plasma. J. Virol. 77: 2214-2226 [Abstract] [Full Text]  
• Santiago, M. L., Bibollet-Ruche, F., Bailes, E., Kamenya, S., Muller, M. N., Lukasik, M., Pusey, A. E., Collins, D. A., Wrangham, R. W., Goodall, J., Shaw, G. M., Sharp, P. M., Hahn, B. H. (2003). Amplification of a Complete Simian Immunodeficiency Virus Genome from Fecal RNA of a Wild Chimpanzee. J. Virol. 77: 2233-2242 [Abstract] [Full Text]  
• Beld, M., Sentjens, R., Rebers, S., Weel, J., Wertheim-van Dillen, P., Sol, C., Boom, R. (2000). Detection and Quantitation of Hepatitis C Virus RNA in Feces of Chronically Infected Individuals. J. Clin. Microbiol. 38: 3442-3444 [Abstract] [Full Text]  
• Liguory, O., David, F., Sarfati, C., Derouin, F., Molina, J.-M. (1998). Determination of Types of Enterocytozoon bieneusi Strains Isolated from Patients with Intestinal Microsporidiosis. J. Clin. Microbiol. 36: 1882-1885 [Abstract] [Full Text]  
• van der Hoek, L, Sol, C., Maas, J, Lukashov, V., Kuiken, C., Goudsmit, J (1998). Genetic differences between human immunodeficiency virus type 1 subpopulations in faeces and serum. J. Gen. Virol. 79: 259-267 [Abstract]