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Journal of Clinical Microbiology, Mar 1995, 629-630, Vol 33, No. 3
Copyright © 1995 by the American Society for Microbiology. All rights reserved.

Inactivation of polymerase inhibitors for Mycobacterium tuberculosis DNA amplification in sputum by using capture resin

M Amicosante, L Richeldi, G Trenti, G Paone, M Campa, A Bisetti and C Saltini
Dipartimento di Scienze Mediche, Oncologiche e Radiologiche, Universita di Modena, Italy.

Endogenous PCR inhibitors hamper DNA amplification of Mycobacterium tuberculosis DNA in sputum samples. Use of an anion binding resin (GeneReleaser [GR]) improved PCR sensitivity from 77% (27 of 35 culture- positive samples) with phenol-chloroform extraction (P-C) alone to 91% (GR without P-C; P > 0.05) and 100% (P-C and GR combined; P < 0.05).


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