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Journal of Clinical Microbiology, Mar 1995, 765-768, Vol 33, No. 3
Copyright © 1995 by the American Society for Microbiology. All rights reserved.

Sensitivity of sandwich enzyme-linked immunosorbent assay for Cryptococcus neoformans polysaccharide antigen is dependent on the isotypes of the capture and detection antibodies

S Mukherjee and A Casadevall
Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10461, USA.

Immunoglobulin M (IgM) and IgG1 monoclonal antibody isotype switch variants to Cryptococcus neoformans capsular polysaccharide were used to study the sensitivity of a double sandwich enzyme-linked immunosorbent assay (ELISA). The most sensitive ELISA configurations used IgG1 monoclonal antibody absorbed on polystyrene plates or IgM immobilized with goat antisera for antigen capture.

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