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Journal of Clinical Microbiology, 03 1995, 775-777, Vol 33, No. 3
Copyright © 1995 by the American Society for Microbiology. All rights reserved.

Development of nested PCR based on the ViaB sequence to detect Salmonella typhi [published erratum appears in J Clin Microbiol 1995 Nov;33(11):3082]

Y Hashimoto, Y Itho, Y Fujinaga, AQ Khan, F Sultana, M Miyake, K Hirose, H Yamamoto and T Ezaki
Department of Microbiology, Gifu University School of Medicine, Japan.

For a rapid diagnosis of typhoid fever, we developed a nested PCR based on the nucleotide sequence encoding the Vi antigen. All Salmonella typhi strains along with a Salmonella paratyphi C strain were PCR positive. This assay was able to detect S. typhi at the single-cell level.

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