This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Johnson, S. R.
Right arrow Articles by Morse, S. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Johnson, S. R.
Right arrow Articles by Morse, S. A.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, Apr 1995, 1036-1038, Vol 33, No. 4
Copyright © 1995 by the American Society for Microbiology. All rights reserved.

Alterations in sample preparation increase sensitivity of PCR assay for diagnosis of chancroid

SR Johnson, DH Martin, C Cammarata and SA Morse
Division of Sexually Transmitted Disease Laboratory Research, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.

A PCR assay for the detection of Haemophilus ducreyi in clinical specimens taken from genital ulcers was developed. Although H. ducreyi, when present in such specimens, could be detected by PCR, the sensitivity of the assay was reduced by the presence of Taq polymerase inhibitors in the specimen. The sensitivity of the PCR assay was improved by the use of detergents in preparing nuclei acids from clinical specimens and by the inclusion of a dialysis step prior to amplification. In addition, sodium phosphate included in the transport medium was found to be an inhibitor of the Taq polymerase.


This article has been cited by other articles:

  • Kuboki, N., Inoue, N., Sakurai, T., Di Cello, F., Grab, D. J., Suzuki, H., Sugimoto, C., Igarashi, I. (2003). Loop-Mediated Isothermal Amplification for Detection of African Trypanosomes. J. Clin. Microbiol. 41: 5517-5524 [Abstract] [Full Text]  
  • Patterson, K., Olsen, B., Thomas, C., Norn, D., Tam, M., Elkins, C. (2002). Development of a Rapid Immunodiagnostic Test for Haemophilus ducreyi. J. Clin. Microbiol. 40: 3694-3702 [Abstract] [Full Text]  
  • Elkins, C., Yi, K., Olsen, B., Thomas, C., Thomas, K., Morse, S. (2000). Development of a Serological Test for Haemophilus ducreyi for Seroprevalence Studies. J. Clin. Microbiol. 38: 1520-1526 [Abstract] [Full Text]  
  • Lewis, D. A (2000). Diagnostic tests for chancroid. Sex. Transm. Infect. 76: 137-141 [Full Text]  
  • Conrads, G., Flemmig, T. F., Seyfarth, I., Lampert, F., Lütticken, R. (1999). Simultaneous Detection of Bacteroides forsythus and Prevotella intermedia by 16S rRNA Gene-Directed Multiplex PCR. J. Clin. Microbiol. 37: 1621-1624 [Abstract] [Full Text]  
  • Matto, J, Saarela, M, Alaluusua, S, Oja, V, Jousimies-Somer, H, Asikainen, S (1998). Detection of Porphyromonas gingivalis from saliva by PCR by using a simple sample-processing method [In Process Citation]. J. Clin. Microbiol. 36: 157-160 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»