This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bassiri, M.
Right arrow Articles by Mardh, P. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bassiri, M.
Right arrow Articles by Mardh, P. A.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, 04 1995, 898-900, Vol 33, No. 4
Copyright © 1995 by the American Society for Microbiology. All rights reserved.

Detection of Chlamydia trachomatis in urine specimens from women by ligase chain reaction

M Bassiri, HY Hu, MA Domeika, J Burczak, LO Svensson, HH Lee and PA Mardh
Institute of Clinical Bacteriology, University of Uppsala, Sweden.

The performance of a plasmid-based ligase chain reaction (LCR) with urine specimens was compared with those of cell culture of cervical swabs and enzyme immunoassay with urine specimens for the detection of Chlamydia trachomatis infection in women who had attended a family planning clinic. The prevalence of chlamydial infection determined by LCR was 3.1%. Discrepant results among the three assays were resolved by testing urine by a second LCR assay based on the C. trachomatis chromosomal gene encoding the major outer membrane protein. Sensitivity, specificity, and positive and negative predictive values for the cell cultures were 56.3, 100, 100, and 98.4%, respectively, whereas those for the enzyme immunoassay were 18.8, 100, 100, and 97.1%, respectively, and those for LCR were 87.5, 100, 100, and 99.5%, respectively. LCR thus provides a highly sensitive and specific noninvasive screening method for detecting genital chlamydial infections in women.


This article has been cited by other articles:

  • Fisher, L. D., Fletcher, K. E., Blake, D. R. (2004). Can the Diagnosis of Pelvic Inflammatory Disease be Excluded Without a Bimanual Examination?. CLIN PEDIATR 43: 153-158 [Abstract]  
  • Blake, D. R., Kearney, M. H., Oakes, J. M., Druker, S. K., Bibace, R. (2003). Improving Participation in Chlamydia Screening Programs: Perspectives of High-Risk Youth. Arch Pediatr Adolesc Med 157: 523-529 [Abstract] [Full Text]  
  • WATSON, E. J., TEMPLETON, A., RUSSELL, I., PAAVONEN, J., MARDH, P.-A., STARY, A., PEDERSON, B. S. (2002). The accuracy and efficacy of screening tests for Chlamydia trachomatis: a systematic review. J Med Microbiol 51: 1021-1031 [Abstract] [Full Text]  
  • Bianchi, A., Moret, F., Desrues, J.-M., Champenois, T., Dervaux, Y., Desvouas, O., Oursin, A., Quinzat, D., Dachez, R., Bathelier, C., Ronsin, C. (2002). PreservCyt Transport Medium Used for the ThinPrep Pap Test Is a Suitable Medium for Detection of Chlamydia trachomatis by the COBAS Amplicor CT/NG Test: Results of a Preliminary Study and Future Implications. J. Clin. Microbiol. 40: 1749-1754 [Abstract] [Full Text]  
  • Akduman, D., Ehret, J. M., Messina, K., Ragsdale, S., Judson, F. N. (2002). Evaluation of a Strand Displacement Amplification Assay (BD ProbeTec-SDA) for Detection of Neisseria gonorrhoeae in Urine Specimens. J. Clin. Microbiol. 40: 281-283 [Abstract] [Full Text]  
  • Van Der Pol, B., Quinn, T. C., Gaydos, C. A., Crotchfelt, K., Schachter, J., Moncada, J., Jungkind, D., Martin, D. H., Turner, B., Peyton, C., Jones, R. B. (2000). Multicenter Evaluation of the AMPLICOR and Automated COBAS AMPLICOR CT/NG Tests for Detection of Chlamydia trachomatis. J. Clin. Microbiol. 38: 1105-1112 [Abstract] [Full Text]  
  • Lauderdale, T.-L., Landers, L., Thorneycroft, I., Chapin, K. (1999). Comparison of the PACE 2 Assay, Two Amplification Assays, and Clearview EIA for Detection of Chlamydia trachomatis in Female Endocervical and Urine Specimens. J. Clin. Microbiol. 37: 2223-2229 [Abstract] [Full Text]  
  • Vincelette, J., Schirm, J., Bogard, M., Bourgault, A.-M., Luijt, D. S., Bianchi, A., van Voorst Vader, P. C., Butcher, A., Rosenstraus, M. (1999). Multicenter Evaluation of the Fully Automated COBAS AMPLICOR PCR Test for Detection of Chlamydia trachomatis in Urogenital Specimens. J. Clin. Microbiol. 37: 74-80 [Abstract] [Full Text]  
  • Wylie, J. L., Moses, S., Babcock, R., Jolly, A., Giercke, S., Hammond, G. (1998). Comparative Evaluation of Chlamydiazyme, PACE 2, and AMP-CT Assays for Detection of Chlamydia trachomatis in Endocervical Specimens. J. Clin. Microbiol. 36: 3488-3491 [Abstract] [Full Text]  
  • Blake, D. R., Duggan, A., Quinn, T., Zenilman, J., Joffe, A. (1998). Evaluation of Vaginal Infections in Adolescent Women: Can It Be Done Without a Speculum?. Pediatrics 102: 939-944 [Abstract] [Full Text]  
  • Carroll, K. C., Aldeen, W. E., Morrison, M., Anderson, R., Lee, D., Mottice, S. (1998). Evaluation of the Abbott LCx Ligase Chain Reaction Assay for Detection of Chlamydia trachomatis and Neisseria gonorrhoeae in Urine and Genital Swab Specimens from a Sexually Transmitted Disease Clinic Population. J. Clin. Microbiol. 36: 1630-1633 [Abstract] [Full Text]  
  • Gaydos, C. A., Howell, M. R., Quinn, T. C., Gaydos, J. C., McKee, K. T. Jr. (1998). Use of Ligase Chain Reaction with Urine versus Cervical Culture for Detection of Chlamydia trachomatis in an Asymptomatic Military Population of Pregnant and Nonpregnant Females Attending Papanicolaou Smear Clinics. J. Clin. Microbiol. 36: 1300-1304 [Abstract] [Full Text]  
  • Green, T. A., Black, C. M., Johnson, R. E. (1998). Evaluation of Bias in Diagnostic-Test Sensitivity and Specificity Estimates Computed by Discrepant Analysis. J. Clin. Microbiol. 36: 375-381 [Abstract] [Full Text]  
  • Crotchfelt, K. A., Pare, B., Gaydos, C., Quinn, T. C. (1998). Detection of Chlamydia trachomatis by the Gen-Probe AMPLIFIED Chlamydia Trachomatis Assay (AMP CT) in Urine Specimens from Men and Women and Endocervical Specimens from Women. J. Clin. Microbiol. 36: 391-394 [Abstract] [Full Text]  
  • Rosenstraus, M, Wang, Z, Chang, S., DeBonville, D, Spadoro, J. (1998). An internal control for routine diagnostic PCR: design, properties, and effect on clinical performance [In Process Citation]. J. Clin. Microbiol. 36: 191-197 [Abstract] [Full Text]  
  • Ostergaard, L., Moller, J. K, Andersen, B., Olesen, F. (1996). Diagnosis of urogenital Chlamydia trachomatis infection in women based on mailed samples obtained at home: multipractice comparative study. BMJ 313: 1186-1189 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»