This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tiballi, R. N.
Right arrow Articles by Kauffman, C. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tiballi, R. N.
Right arrow Articles by Kauffman, C. A.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, 04 1995, 915-917, Vol 33, No. 4
Copyright © 1995 by the American Society for Microbiology. All rights reserved.

Use of a colorimetric system for yeast susceptibility testing

RN Tiballi, X He, LT Zarins, SG Revankar and CA Kauffman
Department of Internal Medicine, Veterans Affairs Medical Center, Ann Arbor, Michigan, USA.

We examined the reliability and accuracy of a colorimetric assay using Alamar Blue reagent in the performance of susceptibility tests for Candida albicans. We compared the broth macrodilution method recommended by the National Committee for Clinical Laboratory Standards (NCCLS) with a macrodilution method modified with the Alamar reagent and a microdilution method modified with the Alamar reagent. The MICs of fluconazole and itraconazole for 97 isolates of C. albicans and 3 control isolates were tested. For fluconazole, the Alamar-modified broth macrodilution method yielded 94% (91 of 97) concordance within 2 dilutions compared with the NCCLS method, while the microdilution method yielded 95% (92 of 97) concordance. With Alamar-modified methods for itraconazole, broth macrodilution yielded 97% (94 of 97) concordance within 2 dilutions. MICs obtained by the microdilution method, although tightly nested, were shifted to a higher value when compared with those obtained by the NCCLS method; there was only 77% (75 of 97) concordance within 2 dilutions but 97% concordance (94 of 97) within 3 dilutions. Tests by all methods with quality control strains showed excellent reproducibilities. For fluconazole, the methods modified with the Alamar reagent yielded clear endpoints and excellent correlation for the broth macrodilution and microdilution methods. For itraconazole, the methods modified with the Alamar reagent yielded clear endpoints and were reproducible, but higher MICs were obtained by the microdilution methods compared with those obtained by the NCCLS methods.


This article has been cited by other articles:

  • Kitamura, A., Higuchi, S., Hata, M., Kawakami, K., Yoshida, K., Namba, K., Nakajima, R. (2009). Effect of {beta}-1,6-Glucan Inhibitors on the Invasion Process of Candida albicans: Potential Mechanism of Their In Vivo Efficacy. Antimicrob. Agents Chemother. 53: 3963-3971 [Abstract] [Full Text]  
  • Kitamura, A., Someya, K., Hata, M., Nakajima, R., Takemura, M. (2009). Discovery of a Small-Molecule Inhibitor of {beta}-1,6-Glucan Synthesis. Antimicrob. Agents Chemother. 53: 670-677 [Abstract] [Full Text]  
  • Khot, P. D., Suci, P. A., Miller, R. L., Nelson, R. D., Tyler, B. J. (2006). A Small Subpopulation of Blastospores in Candida albicans Biofilms Exhibit Resistance to Amphotericin B Associated with Differential Regulation of Ergosterol and {beta}-1,6-Glucan Pathway Genes. Antimicrob. Agents Chemother. 50: 3708-3716 [Abstract] [Full Text]  
  • Acuner, I. C., Eroglu, C., Yilmaz Coban, A., Bozdogan, B., Durupinar, B., Appelbaum, P. C. (2006). Unacceptable Performance and the Lack of Reproducibility Results in the Report of Colorimetric Methods for Early Detection of Vancomycin and Oxacillin Resistance in Staphylococcus aureus.. J. Clin. Microbiol. 44: 2318-2319 [Full Text]  
  • Oda, M. N., Hargreaves, P. L., Beckstead, J. A., Redmond, K. A., van Antwerpen, R., Ryan, R. O. (2006). Reconstituted high density lipoprotein enriched with the polyene antibiotic amphotericin B. J. Lipid Res. 47: 260-267 [Abstract] [Full Text]  
  • Roux, J., Kawakatsu, H., Gartland, B., Pespeni, M., Sheppard, D., Matthay, M. A., Canessa, C. M., Pittet, J.-F. (2005). Interleukin-1{beta} Decreases Expression of the Epithelial Sodium Channel {alpha}-Subunit in Alveolar Epithelial Cells via a p38 MAPK-dependent Signaling Pathway. J. Biol. Chem. 280: 18579-18589 [Abstract] [Full Text]  
  • McBride, J., Ingram, P. R., Henriquez, F. L., Roberts, C. W. (2005). Development of Colorimetric Microtiter Plate Assay for Assessment of Antimicrobials against Acanthamoeba. J. Clin. Microbiol. 43: 629-634 [Abstract] [Full Text]  
  • Romero-Steiner, S., Spear, W., Brown, N., Holder, P., Hennessy, T., Gomez de Leon, P., Carlone, G. M. (2004). Measurement of Serum Bactericidal Activity Specific for Haemophilus influenzae Type b by Using a Chromogenic and Fluorescent Metabolic Indicator. CVI 11: 89-93 [Abstract] [Full Text]  
  • Meletiadis, J., Mouton, J. W., Meis, J. F. G. M., Bouman, B. A., Verweij, P. E. (2002). Comparison of the Etest and the Sensititre Colorimetric Methods with the NCCLS Proposed Standard for Antifungal Susceptibility Testing of Aspergillus Species. J. Clin. Microbiol. 40: 2876-2885 [Abstract] [Full Text]  
  • Pujol, I., Capilla, J., Fernandez-Torres, B., Ortoneda, M., Guarro, J. (2002). Use of the Sensititre Colorimetric Microdilution Panel for Antifungal Susceptibility Testing of Dermatophytes. J. Clin. Microbiol. 40: 2618-2621 [Abstract] [Full Text]  
  • Hawser, S. P., Jessup, C., Vitullo, J., Ghannoum, M. A. (2001). Utility of 2,3-Bis(2-Methoxy-4-Nitro-5-Sulfophenyl)-5-[(Phenyl-Amino)Carbonyl]-2H-Tetrazolium Hydroxide (XTT) and Minimum Effective Concentration Assays in the Determination of Antifungal Susceptibility of Aspergillus fumigatus to the Lipopeptide Class of Compounds. J. Clin. Microbiol. 39: 2738-2741 [Abstract] [Full Text]  
  • Chang, H. C., Chang, J. J., Chan, S. H., Huang, A. H., Wu, T. L., Lin, M. C., Chang, T. C. (2001). Evaluation of Etest for Direct Antifungal Susceptibility Testing of Yeasts in Positive Blood Cultures. J. Clin. Microbiol. 39: 1328-1333 [Abstract] [Full Text]  
  • Chang Chang, H., Jung Chang, J., Huey Huang, A., Chain Chang, T. (2000). Evaluation of a Capacitance Method for Direct Antifungal Susceptibility Testing of Yeasts in Positive Blood Cultures. J. Clin. Microbiol. 38: 971-976 [Abstract] [Full Text]  
  • Okeke, C. N., Tsuboi, R., Kawai, M., Ogawa, H. (2000). Fluorometric Assessment of In Vitro Antidermatophytic Activities of Antimycotics Based on Their Keratin-Penetrating Power. J. Clin. Microbiol. 38: 489-491 [Abstract] [Full Text]  
  • Espinel-Ingroff, A., Pfaller, M., Messer, S. A., Knapp, C. C., Killian, S., Norris, H. A., Ghannoum, M. A. (1999). Multicenter Comparison of the Sensititre YeastOne Colorimetric Antifungal Panel with the National Committee for Clinical Laboratory Standards M27-A Reference Method for Testing Clinical Isolates of Common and Emerging Candida spp., Cryptococcus spp., and Other Yeasts and Yeast-Like Organisms. J. Clin. Microbiol. 37: 591-595 [Abstract] [Full Text]  
  • Pfaller, M. A., Messer, S. A., Karlsson, A., Bolmström, A. (1998). Evaluation of the Etest Method for Determining Fluconazole Susceptibilities of 402 Clinical Yeast Isolates by Using Three Different Agar Media. J. Clin. Microbiol. 36: 2586-2589 [Abstract] [Full Text]  
  • Pfaller, M. A., Arikan, S., Lozano-Chiu, M., Chen, Y.-S., Coffman, S., Messer, S. A., Rennie, R., Sand, C., Heffner, T., Rex, J. H., Wang, J., Yamane, N. (1998). Clinical Evaluation of the ASTY Colorimetric Microdilution Panel for Antifungal Susceptibility Testing. J. Clin. Microbiol. 36: 2609-2612 [Abstract] [Full Text]  
  • Hawser, S. P., Norris, H., Jessup, C. J., Ghannoum, M. A. (1998). Comparison of a 2,3-Bis(2-Methoxy-4-Nitro-5-Sulfophenyl)-5-[(Phenylamino)Carbonyl]-2H-Tetrazolium Hydroxide (XTT) Colorimetric Method with the Standardized National Committee for Clinical Laboratory Standards Method of Testing Clinical Yeast Isolates for Susceptibility to Antifungal Agents. J. Clin. Microbiol. 36: 1450-1452 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»