This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Renneberg, J.
Right arrow Articles by Gutschik, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Renneberg, J.
Right arrow Articles by Gutschik, E.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, May 1995, 1150-1153, Vol 33, No. 5
Copyright © 1995 by the American Society for Microbiology. All rights reserved.

Evaluation of Staph ID 32 system and Staph-Zym system for identification of coagulase-negative staphylococci

J Renneberg, K Rieneck and E Gutschik
Department of Clinical Microbiology, Bispebjerg Hospital, University of Copenhagen, Denmark.

The purpose of the investigation was to evaluate two commercially available identification systems: a new modification of the Staph-Zym system (Rosco, Tastrup, Denmark) and the Staph ID 32 API system (API System, BioMerieux, Paris, France). A local standard method to be used in routine laboratories was also evaluated. A total of 200 staphylococcal isolates, including strains from both the American Type Culture Collection and the Czechoslovak Collection of Microorganisms as well as 89 clinical isolates, were used in tests of all three identification systems. The Staph ID 32 API system identified from 50 to 100% of the reference strains and 82.1% of the clinical isolates correctly. The Staph-Zym system identified from 90 to 100% of the reference strains and 82.1% of the clinical isolates correctly. Most misidentifications were of minor importance, but in both systems major failures appeared (Staphylococcus aureus was identified as a coagulase- negative staphylococcus). Both systems needed backup from a reference laboratory to determine if two isolates were of the same strain.


This article has been cited by other articles:

  • Hauschild, T., Stepanovic, S. (2008). Identification of Staphylococcus spp. by PCR-Restriction Fragment Length Polymorphism Analysis of dnaJ Gene. J. Clin. Microbiol. 46: 3875-3879 [Abstract] [Full Text]  
  • Iorio, N. L. P., Ferreira, R. B. R., Schuenck, R. P., Malvar, K. L., Brilhante, A. P., Nunes, A. P. F., Bastos, C. C. R., dos Santos, K. R. N. (2007). Simplified and Reliable Scheme for Species-Level Identification of Staphylococcus Clinical Isolates. J. Clin. Microbiol. 45: 2564-2569 [Abstract] [Full Text]  
  • Carbonnelle, E., Beretti, J.-L., Cottyn, S., Quesne, G., Berche, P., Nassif, X., Ferroni, A. (2007). Rapid Identification of Staphylococci Isolated in Clinical Microbiology Laboratories by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry. J. Clin. Microbiol. 45: 2156-2161 [Abstract] [Full Text]  
  • Layer, F., Ghebremedhin, B., Moder, K.-A., Konig, W., Konig, B. (2006). Comparative study using various methods for identification of Staphylococcus species in clinical specimens.. J. Clin. Microbiol. 44: 2824-2830 [Abstract] [Full Text]  
  • Shittu, A., Lin, J., Morrison, D., Kolawole, D. (2006). Identification and molecular characterization of mannitol salt positive, coagulase-negative staphylococci from nasal samples of medical personnel and students.. J Med Microbiol 55: 317-324 [Abstract] [Full Text]  
  • Sudagidan, M., Yenidunya, A F., Gunes, H. (2005). Identification of staphylococci by 16S internal transcribed spacer rRNA gene restriction fragment length polymorphism. J Med Microbiol 54: 823-826 [Abstract] [Full Text]  
  • Giammarinaro, P., Leroy, S., Chacornac, J.-P., Delmas, J., Talon, R. (2005). Development of a New Oligonucleotide Array To Identify Staphylococcal Strains at Species Level. J. Clin. Microbiol. 43: 3673-3680 [Abstract] [Full Text]  
  • Heikens, E., Fleer, A., Paauw, A., Florijn, A., Fluit, A. C. (2005). Comparison of Genotypic and Phenotypic Methods for Species-Level Identification of Clinical Isolates of Coagulase-Negative Staphylococci. J. Clin. Microbiol. 43: 2286-2290 [Abstract] [Full Text]  
  • Sakai, H., Procop, G. W., Kobayashi, N., Togawa, D., Wilson, D. A., Borden, L., Krebs, V., Bauer, T. W. (2004). Simultaneous Detection of Staphylococcus aureus and Coagulase-Negative Staphylococci in Positive Blood Cultures by Real-Time PCR with Two Fluorescence Resonance Energy Transfer Probe Sets. J. Clin. Microbiol. 42: 5739-5744 [Abstract] [Full Text]  
  • Becker, K., Harmsen, D., Mellmann, A., Meier, C., Schumann, P., Peters, G., von Eiff, C. (2004). Development and Evaluation of a Quality-Controlled Ribosomal Sequence Database for 16S Ribosomal DNA-Based Identification of Staphylococcus Species. J. Clin. Microbiol. 42: 4988-4995 [Abstract] [Full Text]  
  • Spanu, T., Sanguinetti, M., Ciccaglione, D., D'Inzeo, T., Romano, L., Leone, F., Fadda, G. (2003). Use of the VITEK 2 System for Rapid Identification of Clinical Isolates of Staphylococci from Bloodstream Infections. J. Clin. Microbiol. 41: 4259-4263 [Abstract] [Full Text]  
  • De Paulis, A. N., Predari, S. C., Chazarreta, C. D., Santoianni, J. E. (2003). Five-Test Simple Scheme for Species-Level Identification of Clinically Significant Coagulase-Negative Staphylococci. J. Clin. Microbiol. 41: 1219-1224 [Abstract] [Full Text]  
  • Poyart, C., Quesne, G., Boumaila, C., Trieu-Cuot, P. (2001). Rapid and Accurate Species-Level Identification of Coagulase-Negative Staphylococci by Using the sodA Gene as a Target. J. Clin. Microbiol. 39: 4296-4301 [Abstract] [Full Text]  
  • Edwards, K. J., Kaufmann, M. E., Saunders, N. A. (2001). Rapid and Accurate Identification of Coagulase-Negative Staphylococci by Real-Time PCR. J. Clin. Microbiol. 39: 3047-3051 [Abstract] [Full Text]  
  • Kawamura, Y., Hou, X.-G., Sultana, F., Hirose, K., Miyake, M., Shu, S.-E., Ezaki, T. (1998). Distribution of Staphylococcus Species among Human Clinical Specimens and Emended Description of Staphylococcus caprae. J. Clin. Microbiol. 36: 2038-2042 [Abstract] [Full Text]  
  • Bascomb, S., Manafi, M. (1998). Use of Enzyme Tests in Characterization and Identification of Aerobic and Facultatively Anaerobic Gram-Positive Cocci. Clin. Microbiol. Rev. 11: 318-340 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»