This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Robert, F. Articles by Dupouy-Camet, J. Search for Related Content PubMed PubMed Citation Articles by Robert, F. Articles by Dupouy-Camet, J.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, 09 1995, 2366-2371, Vol 33, No. 9
Copyright © 1995 by the American Society for Microbiology. All rights reserved.

Use of random amplified polymorphic DNA as a typing method for Candida albicans in epidemiological surveillance of a burn unit

F Robert, F Lebreton, ME Bougnoux, A Paugam, D Wassermann, M Schlotterer, C Tourte-Schaefer and J Dupouy-Camet
Laboratoire de Parasitologie, Hopital Cochin, Paris, France.

Burn patients are particularly exposed to deep-seated nosocomial infections caused by Candida species. Superficial carriage of C. albicans is a potential source of infection and dissemination, and typing methods could be useful to trace the different isolates. We report the use of random amplified polymorphic DNA to type isolates of C. albicans in the Hopital Cochin burn unit. This molecular typing method, which is based on PCR with arbitrary short primers, was evaluated on a panel of 32 C. albicans strains isolated from various anatomical sites of unrelated patients, and the strains showed 22 different patterns. Random amplified polymorphic DNA was then used in the epidemiological surveillance of the patients in the burn unit over a 9-month period. Seven patterns were identified among 84 isolates from 18 patients. One pattern (pattern A) corresponding to isolates from 7 of the 18 patients (68% of isolates) predominated throughout the 9- month study, while some strains with other profiles were isolated only once. Some profiles appeared to show a particular geographic pattern within the unit, suggesting transmission from room to room. These results underline the importance of fungal surveillance in such patients and the need to inform nursing staff of measures to prevent the spread of Candida spp. from patient to patient.

This article has been cited by other articles:

• Bougnoux, M.-E., Diogo, D., Francois, N., Sendid, B., Veirmeire, S., Colombel, J. F., Bouchier, C., Van Kruiningen, H., d'Enfert, C., Poulain, D. (2006). Multilocus Sequence Typing Reveals Intrafamilial Transmission and Microevolutions of Candida albicans Isolates from the Human Digestive Tract.. J. Clin. Microbiol. 44: 1810-1820 [Abstract] [Full Text]  
• Viviani, M. A., Cogliati, M., Esposto, M. C., Prigitano, A., Tortorano, A. M. (2006). Four-Year Persistence of a Single Candida albicans Genotype Causing Bloodstream Infections in a Surgical Ward Proven by Multilocus Sequence Typing. J. Clin. Microbiol. 44: 218-221 [Abstract] [Full Text]  
• Bougnoux, M.-E., Morand, S., d'Enfert, C. (2002). Usefulness of Multilocus Sequence Typing for Characterization of Clinical Isolates of Candida albicans. J. Clin. Microbiol. 40: 1290-1297 [Abstract] [Full Text]  
• BECKER, K., BADEHORN, D., DEIWICK, S., PETERS, G., FEGELER, W. (2000). Molecular genotyping of Candida species with special respect to Candida (Torulopsis) glabrata strains by arbitrarily primed PCR. J Med Microbiol 49: 575-581 [Abstract] [Full Text]  
• Soll, D. R. (2000). The Ins and Outs of DNA Fingerprinting the Infectious Fungi. Clin. Microbiol. Rev. 13: 332-370 [Abstract] [Full Text]  
• Marco, F., Lockhart, S. R., Pfaller, M. A., Pujol, C., Rangel-Frausto, M. S., Wiblin, T., Blumberg, H. M., Edwards, J. E., Jarvis, W., Saiman, L., Patterson, J. E., Rinaldi, M. G., Wenzel, R. P., The Nemis Study Group, , Soll, D. R. (1999). Elucidating the Origins of Nosocomial Infections with Candida albicans by DNA Fingerprinting with the Complex Probe Ca3. J. Clin. Microbiol. 37: 2817-2828 [Abstract] [Full Text]  
• Olive, D. M., Bean, P. (1999). Principles and Applications of Methods for DNA-Based Typing of Microbial Organisms. J. Clin. Microbiol. 37: 1661-1669 [Full Text]  
• Bougnoux, M.-E., Dupont, C., Mateo, J., Saulnier, P., Faivre, V., Payen, D., Nicolas-Chanoine, M.-H. (1999). Serum Is More Suitable than Whole Blood for Diagnosis of Systemic Candidiasis by Nested PCR. J. Clin. Microbiol. 37: 925-930 [Abstract] [Full Text]