This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Saito, M.
Right arrow Articles by Kanno, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Saito, M.
Right arrow Articles by Kanno, H.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, 09 1995, 2498-2500, Vol 33, No. 9
Copyright © 1995 by the American Society for Microbiology. All rights reserved.

Immunological detection of penicillin-binding protein 2' of methicillin- resistant staphylococci by using monoclonal antibodies prepared from synthetic peptides

M Saito, K Sekiguchi, R Yajima, M Hina, RC Doss and H Kanno
Research and Development Center, Dainabot Co., Ltd., Chiba, Japan.

Two synthetic peptides 31 and 32 amino acids in length were prepared as deduced from a known amino acid sequence of penicillin-binding protein 2' (PBP2') of methicillin-resistant Staphylococcus aureus. Two monoclonal antibodies were generated from fused cells of myeloma cells and splenic cells of mice immunized with the synthetic peptides. Western blot (immunoblot) analysis demonstrated specific binding of the antibodies to PBP2' of a methicillin-resistant S. aureus strain. An immunoradiometric assay was developed by using these antibodies for simple detection of PBP2'.


This article has been cited by other articles:

  • Wolk, D. M., Johnson, C. H., Rice, E. W., Marshall, M. M., Grahn, K. F., Plummer, C. B., Sterling, C. R. (2000). A Spore Counting Method and Cell Culture Model for Chlorine Disinfection Studies of Encephalitozoon syn. Septata intestinalis. Appl. Environ. Microbiol. 66: 1266-1273 [Abstract] [Full Text]  
  • van Griethuysen, A., Pouw, M., van Leeuwen, N., Heck, M., Willemse, P., Buiting, A., Kluytmans, J. (1999). Rapid Slide Latex Agglutination Test for Detection of Methicillin Resistance in Staphylococcus aureus. J. Clin. Microbiol. 37: 2789-2792 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»