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Journal of Clinical Microbiology, 09 1995, 2511-2513, Vol 33, No. 9
Copyright © 1995 by the American Society for Microbiology. All rights reserved.

Monoclonal antibodies for detection of Norwalk virus antigen in stools

JE Herrmann, NR Blacklow, SM Matsui, TL Lewis, MK Estes, JM Ball and JP Brinker
Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester 01655, USA.

Monoclonal antibodies against the prototype 8FIIa strain of Norwalk virus were prepared and applied to an enzyme immunoassay (EIA) for detecting Norwalk virus in stool specimens. The monoclonal antibodies immunoprecipitated a 58-kDa protein which had been produced by in vitro transcription-translation of Norwalk virus cloned cDNA, and they reacted by EIA with recombinant Norwalk virus capsid protein at a sensitivity level of 1 ng/ml. The EIA detected virus in all tested samples from 15 different Norwalk virus-infected volunteers. No cross- reactions were seen in stools containing other caliciviruses or in stools containing rotaviruses, astroviruses, or enteric adenoviruses.


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