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Journal of Clinical Microbiology, Jan 1996, 38-40, Vol 34, No. 1
Copyright © 1996 by the American Society for Microbiology. All rights reserved.

Comparative evaluation of several techniques for purification of Cryptosporidium parvum oocysts from rat feces

P Suresh and JE Rehg
Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee 38101, USA. suresh@mbcf.stjude.org

Three methods of isolating Cryptosporidium parvum oocysts from rat feces were evaluated. Oocysts were initially isolated by sucrose density gradient centrifugation. They were further purified by passage through a glass bead column or a Percoll gradient or by dialysis. Although oocysts recovered from the glass bead column and by dialysis were relatively free of fecal debris, only oocysts recovered from the Percoll gradient were free of bacteria. Recovered oocysts retained their antigenicity and infectivity. The ability to effectively recover oocysts from rat feces suggests that the laboratory rat may be a convenient substitute for ruminants in the propagation and maintenance of C. parvum oocysts for in vitro and in vivo use.


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