This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Furuta, T. Articles by Futami, H. Search for Related Content PubMed PubMed Citation Articles by Furuta, T. Articles by Futami, H.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, 10 1996, 2421-2425, Vol 34, No. 10
Copyright © 1996 by the American Society for Microbiology. All rights reserved.

Quantitative study of Helicobacter pylori in gastric mucus by competitive PCR using synthetic DNA fragments

T Furuta, E Kaneko, M Suzuki, H Arai and H Futami
First Department of Medicine, Hamamatsu University School of Medicine, Japan.

Helicobacter pylori is closely related to upper gastrointestinal diseases, and the precise evaluation of H. pylori infection is necessary for the treatment of these diseases. The aim of the present study was to establish a method for the quantitative detection of H. pylori. We applied a competitive PCR method using various amounts of synthetic DNA fragments containing the same primer-binding and a subset of the same template sequences as the target competing for primer binding and amplification in order to quantify H. pylori in gastric mucus. The results obtained by this method were compared with the results of histological examination, the rapid urease test, bacterial culture, the [13C]urea breath test, and urea and ammonia measurements in gastric juice. As the quantity of H. pylori in gastric mucus increased, the rates of positivity of histological examination, the rapid urease test, and bacterial culture increased. The quantity of H. pylori in gastric mucus was also significantly correlated with the results of the [13C]urea breath test and was negatively correlated with the urea/ammonia ratio in gastric juice. The competitive PCR method provides an objective measure of the quantity of H. pylori and makes it possible to distinguish true negatives from false negatives due to incomplete PCR and true positives from false positives due to contamination. This method is very useful for the precise evaluation of gastric H. pylori infection.

This article has been cited by other articles:

• Megraud, F., Lehours, P. (2007). Helicobacter pylori Detection and Antimicrobial Susceptibility Testing. Clin. Microbiol. Rev. 20: 280-322 [Abstract] [Full Text]  
• He, Q., Wang, J.-P., Osato, M., Lachman, L. B. (2002). Real-Time Quantitative PCR for Detection of Helicobacter pylori. J. Clin. Microbiol. 40: 3720-3728 [Abstract] [Full Text]  
• Rota, C. A., Pereira-Lima, J. C., Blaya, C., Nardi, N. B. (2001). Consensus and Variable Region PCR Analysis of Helicobacter pylori 3' Region of cagA Gene in Isolates from Individuals with or without Peptic Ulcer. J. Clin. Microbiol. 39: 606-612 [Abstract] [Full Text]  
• Song, Q, Haller, B, Ulrich, D, Wichelhaus, A, Adler, G, Bode, G (2000). Quantitation of Helicobacter pylori in dental plaque samples by competitive polymerase chain reaction. J. Clin. Pathol. 53: 218-222 [Abstract] [Full Text]  
• Matsuoka, M, Yoshida, Y, Hayakawa, K, Fukuchi, S, Sugano, K (1999). Simultaneous colonisation of Helicobacter pylori with and without mutations in the 23S rRNA gene in patients with no history of clarithromycin exposure. Gut 45: 503-507 [Abstract] [Full Text]