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Journal of Clinical Microbiology, 10 1996, 2440-2443, Vol 34, No. 10
Copyright © 1996 by the American Society for Microbiology. All rights reserved.

PCR ribotyping for characterizing Salmonella isolates of different serotypes

C Lagatolla, L Dolzani, E Tonin, A Lavenia, M Di Michele, T Tommasini and C Monti- Bragadin
Dipartimento di Scienze Biomediche, Universita degli Studi, Trieste, Italy.

The 16S-23S intergenic spacer region in 218 strains of Salmonella isolated from four Italian hospitals during the period from 1977 to 1994 was analyzed by PCR ribotyping. This molecular typing technique allowed for the identification of seven different and specific electrophoretic profiles for the seven serovars S: enteritidis, S. london, S. anatum, S. panama, S. heidelberg, S. agona, and S. goldcoast. Otherwise, the spacer region appears to be polymorphic in S. typhimurium S. infantis, and S. derby since we could identify eight, six, and four different ribotypes, respectively.


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