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Journal of Clinical Microbiology, Oct 1996, 2454-2459, Vol 34, No. 10
Copyright © 1996 by the American Society for Microbiology. All rights reserved.

Direct identification and typing of Mycobacterium tuberculosis by PCR

H Neimark, M Ali Baig and S Carleton
Department of Microbiology and Immunology, College of Medicine, State University of New York at Brooklyn 11203-2098, USA.

We have developed a rapid PCR assay that types strains of Mycobacterium tuberculosis by generating distinct DNA fingerprints directly from primary cultures. This assay allows strain identification analogous to that achieved by the standard restriction fragment length polymorphism method, and fingerprints are obtained in less than 8 h. This assay does not require subculturing, DNA purification, restriction digestion, Southern blotting, or nucleic acid hybridization. Rapid and precise identification of M. tuberculosis strains permits immediate molecular epidemiologic studies. The assay can be converted to a computer- automated system by employing fluorescently labeled PCR primers and the Perkin-Elmer DNA sequencer so that unknown-specimen fingerprints are identified by computer comparison to a database of M. tuberculosis strain fingerprints.


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