This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Skuce, R. A.
Right arrow Articles by Neill, S. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Skuce, R. A.
Right arrow Articles by Neill, S. D.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, 10 1996, 2469-2474, Vol 34, No. 10
Copyright © 1996 by the American Society for Microbiology. All rights reserved.

Differentiation of Mycobacterium bovis isolates from animals by DNA typing

RA Skuce, D Brittain, MS Hughes and SD Neill
Department of Agriculture for Northern Ireland, Belfast, United Kingdom. RSKUCE@QUB.AC.UK

The insertion sequence IS6110 and the direct repeat (DR) specific to tuberculosis complex mycobacteria and the highly repeated DNA sequence, the polymorphic GC-rich repeat sequence (PGRS), were systematically used to identify restriction fragment length polymorphisms (RFLPs) within 210 isolates of Mycobacterium bovis. The isolates were primarily of bovine origin, but isolates from badgers, feral deer, sheep, humans, and a pig were included. The RFLP probes IS6110, DR, and PGRS individually identified 17, 18, and 18 different RFLP types, respectively, but in combination these probes identified a total of 39 different M. bovis RFLP types. The recommendations (J. D. A. van Embden, M. D. Cave, J. T. Crawford, J. W. Dale, K. D. Eisenach, B. Gicquel, P. W. M. Hermans, C. Martin, R. McAdam, T. M. Shinnick, and P. M. Small, J. Clin. Microbiol. 31:406-409, 1993) for a standardized RFLP analysis for M. tuberculosis were adapted to facilitate gel documentation, image analysis, and construction of a database of RFLP types. In the present study the same M. bovis RFLP types were evident in the various animal species included, indicating that the strains were not host restricted. Application of these techniques to defined field studies should help elucidate more accurately aspects of the epidemiology of bovine tuberculosis in different countries.


This article has been cited by other articles:

  • Skuce, R. A., McCorry, T. P., McCarroll, J. F., Roring, S. M. M., Scott, A. N., Brittain, D., Hughes, S. L., Hewinson, R. G., Neill, S. D. (2002). Discrimination of Mycobacterium tuberculosis complex bacteria using novel VNTR-PCR targets. Microbiology 148: 519-528 [Abstract] [Full Text]  
  • Taylor, M. J., Hughes, M. S., Skuce, R. A., Neill, S. D. (2001). Detection of Mycobacterium bovis in Bovine Clinical Specimens Using Real-Time Fluorescence and Fluorescence Resonance Energy Transfer Probe Rapid-Cycle PCR. J. Clin. Microbiol. 39: 1272-1278 [Abstract] [Full Text]  
  • O'Brien, R., Danilowicz, B. S., Bailey, L., Flynn, O., Costello, E., O'Grady, D., Rogers, M. (2000). Characterization of the Mycobacterium bovis Restriction Fragment Length Polymorphism DNA Probe pUCD and Performance Comparison with Standard Methods. J. Clin. Microbiol. 38: 3362-3369 [Abstract] [Full Text]  
  • O'Brien, R., Flynn, O., Costello, E., O'Grady, D., Rogers, M. (2000). Identification of a Novel DNA Probe for Strain Typing Mycobacterium bovis by Restriction Fragment Length Polymorphism Analysis. J. Clin. Microbiol. 38: 1723-1730 [Abstract] [Full Text]  
  • Park, Y.-K., Bai, G.-H., Kim, S.-J. (2000). Restriction Fragment Length Polymorphism Analysis of Mycobacterium tuberculosis Isolated from Countries in the Western Pacific Region. J. Clin. Microbiol. 38: 191-197 [Abstract] [Full Text]  
  • Espitia, C., Laclette, J. P., Mondragon- Palomino, M., Amador, A., Campuzano, J., Martens, A., Singh, M., Cicero, R., Zhang, Y., Moreno, C. (1999). The PE-PGRS glycine-rich proteins of Mycobacterium tuberculosis : a new family of fibronectin-binding proteins?. Microbiology 145: 3487-3495 [Abstract] [Full Text]  
  • Costello, E., O'Grady, D., Flynn, O., O'Brien, R., Rogers, M., Quigley, F., Egan, J., Griffin, J. (1999). Study of Restriction Fragment Length Polymorphism Analysis and Spoligotyping for Epidemiological Investigation of Mycobacterium bovis Infection. J. Clin. Microbiol. 37: 3217-3222 [Abstract] [Full Text]  
  • Serraino, A., Marchetti, G., Sanguinetti, V., Rossi, M. C., Zanoni, R. G., Catozzi, L., Bandera, A., Dini, W., Mignone, W., Franzetti, F., Gori, A. (1999). Monitoring of Transmission of Tuberculosis between Wild Boars and Cattle: Genotypical Analysis of Strains by Molecular Epidemiology Techniques. J. Clin. Microbiol. 37: 2766-2771 [Abstract] [Full Text]  
  • Zumárraga, M. J., Martin, C., Samper, S., Alito, A., Latini, O., Bigi, F., Roxo, E., Cicuta, M. E., Errico, F., Ramos, M. C., Cataldi, A., van Soolingen, D., Romano, M. I. (1999). Usefulness of Spoligotyping in Molecular Epidemiology of Mycobacterium bovis-Related Infections in South America. J. Clin. Microbiol. 37: 296-303 [Abstract] [Full Text]  
  • Cousins, D, Williams, S, Liebana, E, Aranaz, A, Bunschoten, A, Van Embden, J, Ellis, T (1998). Evaluation of four DNA typing techniques in epidemiological investigations of bovine tuberculosis [In Process Citation]. J. Clin. Microbiol. 36: 168-178 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»