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Journal of Clinical Microbiology, Nov 1996, 2812-2814, Vol 34, No. 11
Copyright © 1996 by the American Society for Microbiology. All rights reserved.

Rapid detection and isolation of shiga-like toxin (verocytotoxin)- producing Escherichia coli by direct testing of individual enterohemolytic colonies from washed sheep blood agar plates in the VTEC-RPLA assay

L Beutin, S Zimmermann and K Gleier
Department of Microbiology, Robert Koch-Institut, Berlin, Germany.

By combining the enterohemolysin test and the VTEC-RPLA test (specific for the detection of Shiga-like toxin I [SLT-I], SLT-II, and SLT-IIc), single colonies of SLT-producing Escherichia coli were found to constitute between 0.03 and 68.1% of the coliform flora in human stool cultures and were isolated and characterized within 72 to 96 h.


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