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Journal of Clinical Microbiology, Dec 1996, 3230-3232, Vol 34, No. 12
Copyright © 1996 by the American Society for Microbiology. All rights reserved.

Detection of the microsporidian parasite Enterocytozoon bieneusi in specimens from patients with AIDS by PCR

JN Velasquez, S Carnevale, EA Guarnera, JH Labbe, A Chertcoff, MG Cabrera and MI Rodriguez
Department of Parasitology, National Institute of Microbiology, Dr. Carlos G. Malbran, Buenos Aires, Argentina. parasito@malbra.sld.ar

Microsporidia are protozoa parasites responsible for significant gastrointestinal disease in patients infected with human immunodeficiency virus. We evaluated a PCR assay of stool samples, duodenal aspirates, and biopsy specimens from patients with Enterocytozoon bieneusi infection. A 210-bp DNA fragment of the unique rRNA intergenic spacer could be amplified from all samples infected with E. bieneusi, but no amplification was seen by using DNA purified from samples with Septata intestinalis or other parasites and from negative control human cells. These results suggest that the PCR in stool samples may be a useful tool for the diagnosis of intestinal microsporidiosis in patients with AIDS.

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