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Journal of Clinical Microbiology, 02 1996, 466-470, Vol 34, No. 2
Copyright © 1996 by the American Society for Microbiology. All rights reserved.

Serotyping of Cryptococcus neoformans by dot enzyme assay

T Belay, R Cherniak, EB O'Neill and TR Kozel
Department of Chemistry, Georgia State University, Atlanta 30303, USA.

A method is described for the serotyping of Cryptococcus neoformans based on direct analysis of culture supernatants for the major type- specific capsular antigen, glucuronoxylomannan. Factor sera prepared by absorption of polyclonal rabbit antisera (Iatron Laboratories, Inc., Tokyo, Japan) or selected anti-C. neoformans monoclonal antibodies were used in a dot enzyme assay to detect the presence of antigen.


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