This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hla, S. W. Articles by Ho, B. Search for Related Content PubMed PubMed Citation Articles by Hla, S. W. Articles by Ho, B.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, 03 1996, 575-578, Vol 34, No. 3
Copyright © 1996 by the American Society for Microbiology. All rights reserved.

Genome macrorestriction analysis of sequential Pseudomonas aeruginosa isolates from bronchiectasis patients without cystic fibrosis

SW Hla, KP Hui, WC Tan and B Ho
Department of Medicine and Department of Microbiology, National University of Singapore.

The respiratory tracts of bronchiectasis patients may be persistently colonized with Pseudomonas aeruginosa, despite intensive chemotherapy. The organism may undergo phenotypic changes in these patients, providing misleading typing results by conventional methods. We prospectively studied eight bronchiectasis patients without cystic fibrosis over a period of 1 year. A high microbial load of P. aeruginosa was found in 70% of sputum samples collected. Of these, 55 sequential P. aeruginosa isolates were characterized by a genotyping method, pulsed-field gel electrophoresis, to overcome the problem of differentiating the P. aeruginosa strains during chemotherapy. Genome macrorestriction fingerprinting patterns were analyzed after digestion with XbaI restriction endonuclease. Of the eight patients, six harbored a single dominant strain of P. aeruginosa, with an intrapatient macrorestriction similarity pattern range of 96 to 100%. The other two patients were infected with mixed bacterial isolates including P. aeruginosa. However, diversity was observed in the P. aeruginosa isolates from all eight patients, with a relatedness of only 55 to 65%. The study further strengthens the fact that pulsed-field gel electrophoresis can be used efficiently and effectively to differentiate P. aeruginosa strains in bronchiectasis patients without cystic fibrosis.

This article has been cited by other articles:

• Macia, M. D., Blanquer, D., Togores, B., Sauleda, J., Perez, J. L., Oliver, A. (2005). Hypermutation Is a Key Factor in Development of Multiple-Antimicrobial Resistance in Pseudomonas aeruginosa Strains Causing Chronic Lung Infections. Antimicrob. Agents Chemother. 49: 3382-3386 [Abstract] [Full Text]  
• Yau, Y. H., Ho, B., Tan, N. S., Ng, M. L., Ding, J. L. (2001). High Therapeutic Index of Factor C Sushi Peptides: Potent Antimicrobials against Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 45: 2820-2825 [Abstract] [Full Text]  
• Pujana, I., Gallego, L., Martín, G., López, F., Canduela, J., Cisterna, R. (1999). Epidemiological Analysis of Sequential Pseudomonas aeruginosa Isolates from Chronic Bronchiectasis Patients without Cystic Fibrosis. J. Clin. Microbiol. 37: 2071-2073 [Abstract] [Full Text]  
• Morrison, D., Woodford, N., Barrett, S. P., Sisson, P., Cookson, B. D. (1999). DNA Banding Pattern Polymorphism in Vancomycin-Resistant Enterococcus faecium and Criteria for Defining Strains. J. Clin. Microbiol. 37: 1084-1091 [Abstract] [Full Text]