This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Connor, K. M. Articles by Donachie, W. Search for Related Content PubMed PubMed Citation Articles by Connor, K. M. Articles by Donachie, W.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, 03 1996, 638-640, Vol 34, No. 3
Copyright © 1996 by the American Society for Microbiology. All rights reserved.

Moredun Bordetella Medium, an improved selective medium for isolation of Bordetella parapertussis

KM Connor, JF Porter, MM Quirie and W Donachie
Moredun Research Institute, Edinburgh, UK.

Bordetella parapertussis, previously thought to be an obligate human respiratory tract pathogen, has been isolated from sheep. Attempts to assess the prevalence of B. parapertussis in conventionally reared sheep by nasal swabbing proved futile with existing selective media because of extensive overgrowth with Mucor spp. and other nasal commensals. Moredun Bordetella Medium (MBM), which contains cycloheximide and spectinomycin at final concentrations of 0.5 mg/ml and 100 mu g/ml, respectively, was developed as an improved selective medium to isolate B. parapertussis from the nasal cavities of conventionally reared sheep. The selective ability of MBM was evaluated with 200 nasal swabs from conventionally reared sheep, and B. parapertussis was recovered from 31.5% of the samples. MBM facilitated the simple and effective isolation of B. parapertussis from ovine nasal swabs and, in successfully excluding overgrowth with other contaminants, proved superior to other test formulations evaluated and to existing conventional media.

This article has been cited by other articles:

• Farrell, D. J., Daggard, G., Mukkur, T. K. S. (1999). Nested Duplex PCR To Detect Bordetella pertussis and Bordetella parapertussis and Its Application in Diagnosis of Pertussis in Nonmetropolitan Southeast Queensland, Australia. J. Clin. Microbiol. 37: 606-610 [Abstract] [Full Text]  
• Allen, A. G., Isobe, T., Maskell, D. J. (1998). Identification and Cloning of waaF (rfaF) from Bordetella pertussis and Use To Generate Mutants of Bordetella spp. with Deep Rough Lipopolysaccharide. J. Bacteriol. 180: 35-40 [Abstract] [Full Text]