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Journal of Clinical Microbiology, 04 1996, 1014-1016, Vol 34, No. 4
Copyright © 1996 by the American Society for Microbiology. All rights reserved.

Molecular study of nosocomial nocardiosis outbreak involving heart transplant recipients

L Exmelin, B Malbruny, M Vergnaud, F Prosvost, P Boiron and C Morel
Laboratoire de Microbiologie, Centre-Hospitalo-Universitaire Cote de Nacre, Caen, France.

Randomly amplified polymorphic DNA analysis and rRNA gene restriction patterns (ribotyping) were compared as methods of investigating a nosocomial outbreak of nocardiosis involving three heart transplant recipients. No clear distinctions between three clinically related isolates and four unrelated strains were obtained by ribotyping. On the contrary, randomly amplified polymorphic DNA analysis with two selected primers, primers 2650 and DKU49, showed one pattern for the three related isolates and four patterns for the unrelated strains.


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