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Journal of Clinical Microbiology, Apr 1996, 953-958, Vol 34, No. 4
Copyright © 1996 by the American Society for Microbiology. All rights reserved.
Sequencing emm-specific PCR products for routine and accurate typing of group A streptococci
B Beall, R Facklam and T Thompson
Childhood and Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
Rapid sequence analysis of specific PCR products was used to accurately
deduce emm types corresponding to the majority of the known group A
streptococcal (GAS) M serotypes. The study involved 95 M type reference GAS
strains and a survey of 74 recent clinical isolates. A high percentage of
agreement between M type serology and the previously published 5' sequences
of the emm genes of M type reference strains was noted. The 5' sequences
for six established M protein genes--the emm- 32, emm-34, emm-38, emm-40,
emm-42, and emm-71 genes--were determined to supplement the existing emm
sequence database. Rapid sequence analysis differentiated serologically
M-nontypeable strains and was used to establish the probable.
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