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Journal of Clinical Microbiology, Jun 1996, 1465-1467, Vol 34, No. 6
Copyright © 1996 by the American Society for Microbiology. All rights reserved.

Detection of enteroviruses from clinical specimens by spin amplification shell vial culture and monoclonal antibody assay

SL Klespies, DE Cebula, CL Kelley, D Galehouse and CC Maurer
Department of Pathology and Laboratory Medicine, Children's Hospital Medical Center of Akron, Ohio 44308, USA.

Conventional tube cell culture was compared with a 72-h, spin-amplified shell vial indirect immunofluorescence assay for the detection of enterovirus from clinical specimens. The sensitivity for the shell vial assay after resolution of discrepant results were 93 and 100%, respectively. The shell vial assay detected 93% of the positive cultures within 72 h of incubation while conventional tube culture detected only 51% of the positive cultures within the same time interval. The data suggest that a spin-amplified shell vial indirect immunofluorescence assay may be useful for the detection of enterovirus from clinical specimens.

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