This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Paton, A. W.
Right arrow Articles by Paton, J. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Paton, A. W.
Right arrow Articles by Paton, J. C.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, Jul 1996, 1622-1627, Vol 34, No. 7
Copyright © 1996 by the American Society for Microbiology. All rights reserved.

Molecular microbiological investigation of an outbreak of hemolytic- uremic syndrome caused by dry fermented sausage contaminated with Shiga- like toxin-producing Escherichia coli

AW Paton, RM Ratcliff, RM Doyle, J Seymour-Murray, D Davos, JA Lanser and JC Paton
Molecular Microbiology Unit, Women's and Children's Hospital, North Adelaide, Australia. patonj@wch.sa.gov.au

Shiga-like toxin-producing Escherichia coli (SLTEC) strains are a diverse group of organisms which are known to cause diarrhea and hemorrhagic colitis in humans. This can lead to potentially fatal systemic sequelae, such as hemolytic-uremic syndrome (HUS). Strains belonging to more than 100 different O:H serotypes have been associated with severe SLTEC disease in humans, of which only O157 strains (which are uncommon in Australia) have a distinguishable cultural characteristic (sorbitol negative). During an outbreak of HUS in Adelaide, South Australia, a sensitive PCR assay specific for Shiga- like toxin genes (slt) was used to test cultures of feces and suspected foods. This enabled rapid confirmation of infection and identified a locally produced dry fermented sausage (mettwurst) as the source of infection. Cultures of feces from 19 of 21 HUS patients and 7 of 8 mettwurst samples collected from their homes were PCR positive for slt- I and slt-II genes. SLTEC isolates belonging to serotype O111:H- was subsequently isolated from 16 patients and 4 mettwurst samples. Subsequent restriction fragment length polymorphism analysis of chromosomal DNA from these isolates with slt-specific probes indicated that at least three different O111:H- genotypes were associated with the outbreak. Pulsed-field gel electrophoresis of genomic DNA restricted with XbaI showed that two of these restriction fragment length polymorphism types were closely related, but the third was quite distinct. However, SLTEC strains of other serotypes, including O157:H-, were also isolated from some of the HUS patients.


This article has been cited by other articles:

  • Gilmour, M. W., Olson, A. B., Andrysiak, A. K., Ng, L.-K., Chui, L. (2007). Sequence-based typing of genetic targets encoded outside of the O-antigen gene cluster is indicative of Shiga toxin-producing Escherichia coli serogroup lineages. J Med Microbiol 56: 620-628 [Abstract] [Full Text]  
  • Vidovic, S., Korber, D. R. (2006). Prevalence of Escherichia coli O157 in Saskatchewan Cattle: Characterization of Isolates by Using Random Amplified Polymorphic DNA PCR, Antibiotic Resistance Profiles, and Pathogenicity Determinants.. Appl. Environ. Microbiol. 72: 4347-4355 [Abstract] [Full Text]  
  • Hiramatsu, R., Matsumoto, M., Sakae, K., Miyazaki, Y. (2005). Ability of Shiga Toxin-Producing Escherichia coli and Salmonella spp. To Survive in a Desiccation Model System and in Dry Foods. Appl. Environ. Microbiol. 71: 6657-6663 [Abstract] [Full Text]  
  • Sheoran, A. S., Chapman-Bonofiglio, S., Harvey, B. R., Mukherjee, J., Georgiou, G., Donohue-Rolfe, A., Tzipori, S. (2005). Human Antibody against Shiga Toxin 2 Administered to Piglets after the Onset of Diarrhea Due to Escherichia coli O157:H7 Prevents Fatal Systemic Complications. Infect. Immun. 73: 4607-4613 [Abstract] [Full Text]  
  • Torres, A. G., Vazquez-Juarez, R. C., Tutt, C. B., Garcia-Gallegos, J. G. (2005). Pathoadaptive Mutation That Mediates Adherence of Shiga Toxin-Producing Escherichia coli O111. Infect. Immun. 73: 4766-4776 [Abstract] [Full Text]  
  • Hornitzky, M. A., Mercieca, K., Bettelheim, K. A., Djordjevic, S. P. (2005). Bovine Feces from Animals with Gastrointestinal Infections Are a Source of Serologically Diverse Atypical Enteropathogenic Escherichia coli and Shiga Toxin-Producing E. coli Strains That Commonly Possess Intimin. Appl. Environ. Microbiol. 71: 3405-3412 [Abstract] [Full Text]  
  • Shaw, D. J., Jenkins, C., Pearce, M. C., Cheasty, T., Gunn, G. J., Dougan, G., Smith, H. R., Woolhouse, M. E. J., Frankel, G. (2004). Shedding Patterns of Verocytotoxin-Producing Escherichia coli Strains in a Cohort of Calves and Their Dams on a Scottish Beef Farm. Appl. Environ. Microbiol. 70: 7456-7465 [Abstract] [Full Text]  
  • Paton, A. W., Srimanote, P., Talbot, U. M., Wang, H., Paton, J. C. (2004). A New Family of Potent AB5 Cytotoxins Produced by Shiga Toxigenic Escherichia coli. JEM 0: jem.20040392--C12 [Abstract] [Full Text]  
  • Cornick, N. A., Helgerson, A. F. (2004). Transmission and Infectious Dose of Escherichia coli O157:H7 in Swine. Appl. Environ. Microbiol. 70: 5331-5335 [Abstract] [Full Text]  
  • Jenkins, C., Willshaw, G. A., Evans, J., Cheasty, T., Chart, H., Shaw, D. J., Dougan, G., Frankel, G., Smith, H. R. (2003). Subtyping of virulence genes in verocytotoxin-producing Escherichia coli (VTEC) other than serogroup O157 associated with disease in the United Kingdom. J Med Microbiol 52: 941-947 [Abstract] [Full Text]  
  • Matussek, A., Lauber, J., Bergau, A., Hansen, W., Rohde, M., Dittmar, K. E. J., Gunzer, M., Mengel, M., Gatzlaff, P., Hartmann, M., Buer, J., Gunzer, F. (2003). Molecular and functional analysis of Shiga toxin-induced response patterns in human vascular endothelial cells. Blood 102: 1323-1332 [Abstract] [Full Text]  
  • Sheoran, A. S., Chapman, S., Singh, P., Donohue-Rolfe, A., Tzipori, S. (2003). Stx2-Specific Human Monoclonal Antibodies Protect Mice against Lethal Infection with Escherichia coli Expressing Stx2 Variants. Infect. Immun. 71: 3125-3130 [Abstract] [Full Text]  
  • Bettelheim, K. A. (2003). Non-O157 Verotoxin-Producing Escherichia coli: A Problem, Paradox, and Paradigm. Exp. Biol. Med. 228: 333-344 [Abstract] [Full Text]  
  • Callaway, T. R., Elder, R. O., Keen, J. E., Anderson, R. C., Nisbet, D. J. (2003). Forage Feeding to Reduce Preharvest Escherichia coli Populations in Cattle, a Review. J DAIRY SCI 86: 852-860 [Abstract] [Full Text]  
  • Bettelheim, K. A., Hornitzky, M. A., Djordjevic, S. P., Kuzevski, A. (2003). Antibiotic resistance among verocytotoxigenic Escherichia coli (VTEC) and non-VTEC isolated from domestic animals and humans. J Med Microbiol 52: 155-162 [Abstract] [Full Text]  
  • Paton, A. W., Paton, J. C. (2002). Reactivity of Convalescent-Phase Hemolytic-Uremic Syndrome Patient Sera with the Megaplasmid-Encoded TagA Protein of Shiga Toxigenic Escherichia coli O157. J. Clin. Microbiol. 40: 1395-1399 [Abstract] [Full Text]  
  • Tarr, C. L., Whittam, T. S. (2002). Molecular Evolution of the Intimin Gene in O111 Clones of Pathogenic Escherichia coli. J. Bacteriol. 184: 479-487 [Abstract] [Full Text]  
  • Eklund, M., Scheutz, F., Siitonen, A. (2001). Clinical Isolates of Non-O157 Shiga Toxin-Producing Escherichia coli: Serotypes, Virulence Characteristics, and Molecular Profiles of Strains of the Same Serotype. J. Clin. Microbiol. 39: 2829-2834 [Abstract] [Full Text]  
  • Elliott, E J, Robins-Browne, R M, O'Loughlin, E V, Bennett-Wood, V, Bourke, J, Henning, P, Hogg, G G, Knight, J, Powell, H, Redmond, D, Contributors to the Australian Paediatric Surveill, (2001). Nationwide study of haemolytic uraemic syndrome: clinical, microbiological, and epidemiological features. Arch. Dis. Child. 85: 125-131 [Abstract] [Full Text]  
  • Paton, J. C., Rogers, T. J., Morona, R., Paton, A. W. (2001). Oral Administration of Formaldehyde-Killed Recombinant Bacteria Expressing a Mimic of the Shiga Toxin Receptor Protects Mice from Fatal Challenge with Shiga-Toxigenic Escherichia coli. Infect. Immun. 69: 1389-1393 [Abstract] [Full Text]  
  • Ogawa, M., Shimizu, K., Nomoto, K., Takahashi, M., Watanuki, M., Tanaka, R., Tanaka, T., Hamabata, T., Yamasaki, S., Takeda, Y. (2001). Protective Effect of Lactobacillus casei Strain Shirota on Shiga Toxin-Producing Escherichia coli O157:H7 Infection in Infant Rabbits. Infect. Immun. 69: 1101-1108 [Abstract] [Full Text]  
  • WILLSHAW, G.A., CHEASTY, T., SMITH, H.R., O'BRIEN, S.J., ADAK, G.K. (2001). Verocytotoxin-producing Escherichia coli (VTEC) O157 and other VTEC from human infections in England and Wales: 1995-1998. J Med Microbiol 50: 135-142 [Abstract] [Full Text]  
  • Kobayashi, H., Shimada, J., Nakazawa, M., Morozumi, T., Pohjanvirta, T., Pelkonen, S., Yamamoto, K. (2001). Prevalence and Characteristics of Shiga Toxin-Producing Escherichia coli from Healthy Cattle in Japan. Appl. Environ. Microbiol. 67: 484-489 [Abstract] [Full Text]  
  • Bellin, T., Pulz, M., Matussek, A., Hempen, H.-G., Gunzer, F. (2001). Rapid Detection of Enterohemorrhagic Escherichia coli by Real-Time PCR with Fluorescent Hybridization Probes. J. Clin. Microbiol. 39: 370-374 [Abstract] [Full Text]  
  • Ogierman, M. A., Paton, A. W., Paton, J. C. (2000). Up-Regulation of Both Intimin and eae-Independent Adherence of Shiga Toxigenic Escherichia coli O157 by ler and Phenotypic Impact of a Naturally Occurring ler Mutation. Infect. Immun. 68: 5344-5353 [Abstract] [Full Text]  
  • Paton, A. W., Woodrow, M. C., Doyle, R. M., Lanser, J. A., Paton, J. C. (1999). Molecular Characterization of a Shiga Toxigenic Escherichia coli O113:H21 Strain Lacking eae Responsible for a Cluster of Cases of Hemolytic-Uremic Syndrome. J. Clin. Microbiol. 37: 3357-3361 [Abstract] [Full Text]  
  • Rios, M., Prado, V., Trucksis, M., Arellano, C., Borie, C., Alexandre, M., Fica, A., Levine, M. M. (1999). Clonal Diversity of Chilean Isolates of Enterohemorrhagic Escherichia coli from Patients with Hemolytic-Uremic Syndrome, Asymptomatic Subjects, Animal Reservoirs, and Food Products. J. Clin. Microbiol. 37: 778-781 [Abstract] [Full Text]  
  • Paton, A. W., Manning, P. A., Woodrow, M. C., Paton, J. C. (1998). Translocated Intimin Receptors (Tir) of Shiga-Toxigenic Escherichia coli Isolates Belonging to Serogroups O26, O111, and O157 React with Sera from Patients with Hemolytic-Uremic Syndrome and Exhibit Marked Sequence Heterogeneity. Infect. Immun. 66: 5580-5586 [Abstract] [Full Text]  
  • Wang, L., Curd, H., Qu, W., Reeves, P. R. (1998). Sequencing of Escherichia coli O111 O-Antigen Gene Cluster and Identification of O111-Specific Genes. J. Clin. Microbiol. 36: 3182-3187 [Abstract] [Full Text]  
  • Paton, J. C., Paton, A. W. (1998). Pathogenesis and Diagnosis of Shiga Toxin-Producing Escherichia coli Infections. Clin. Microbiol. Rev. 11: 450-479 [Abstract] [Full Text]  
  • Bonnet, R., Souweine, B., Gauthier, G., Rich, C., Livrelli, V., Sirot, J., Joly, B., Forestier, C. (1998). Non-O157:H7 Stx2-Producing Escherichia coli Strains Associated with Sporadic Cases of Hemolytic-Uremic Syndrome in Adults. J. Clin. Microbiol. 36: 1777-1780 [Abstract] [Full Text]  
  • Desmarchelier, P. M., Bilge, S. S., Fegan, N., Mills, L., Vary, J. C. Jr., Tarr, P. I. (1998). A PCR Specific for Escherichia coli O157 Based on the rfb Locus Encoding O157 Lipopolysaccharide. J. Clin. Microbiol. 36: 1801-1804 [Abstract] [Full Text]  
  • Uyttendaele, M., Grangette, C., Rogerie, F., Pasteau, S., Debevere, J., Lange, M. (1998). Influence of Cold Stress on the Preliminary Enrichment Time Needed for Detection of Enterohemorrhagic Escherichia coli in Ground Beef by PCR. Appl. Environ. Microbiol. 64: 1640-1643 [Abstract] [Full Text]  
  • Voss, E., Paton, A. W., Manning, P. A., Paton, J. C. (1998). Molecular Analysis of Shiga Toxigenic Escherichia coli O111:H- Proteins Which React with Sera from Patients with Hemolytic-Uremic Syndrome. Infect. Immun. 66: 1467-1472 [Abstract] [Full Text]  
  • Morabito, S., Karch, H., Mariani-Kurkdjian, P., Schmidt, H., Minelli, F., Bingen, E., Caprioli, A. (1998). Enteroaggregative, Shiga Toxin-Producing Escherichia coli O111:H2 Associated with an Outbreak of Hemolytic-Uremic Syndrome. J. Clin. Microbiol. 36: 840-842 [Abstract] [Full Text]  
  • Paton, A. W., Paton, J. C. (1998). Detection and Characterization of Shiga Toxigenic Escherichia coli by Using Multiplex PCR Assays for stx1, stx2, eaeA, Enterohemorrhagic E. coli hlyA, rfbO111, and rfbO157. J. Clin. Microbiol. 36: 598-602 [Abstract] [Full Text]  
  • Nataro, J. P., Kaper, J. B. (1998). Diarrheagenic Escherichia coli. Clin. Microbiol. Rev. 11: 142-201 [Abstract] [Full Text]  
  • Keene, W. E., Sazie, E., Kok, J., Rice, D. H., Hancock, D. D., Balan, V. K., Zhao, T., Doyle, M. P. (1997). An Outbreak of Escherichia coli 0157:H7 Infections Traced to Jerky Made From Deer Meat. JAMA 277: 1229-1231 [Abstract]  
  • Elder, R. O., Keen, J. E., Siragusa, G. R., Barkocy-Gallagher, G. A., Koohmaraie, M., Laegreid, W. W. (2000). From the Cover: Correlation of enterohemorrhagic Escherichia coli O157 prevalence in feces, hides, and carcasses of beef cattle during processing. Proc. Natl. Acad. Sci. USA 97: 2999-3003 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»