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Journal of Clinical Microbiology, 07 1996, 1825-1828, Vol 34, No. 7
Copyright © 1996 by the American Society for Microbiology. All rights reserved.

Molecular epidemiology of Mycobacterium tuberculosis strains isolated from different regions of Italy and Pakistan

LA Sechi, S Zanetti, G Delogu, B Montinaro, A Sanna and G Fadda
Institute of Microbiology and Virology, University of Sassari, Italy. microb@ssmain.uniss.it

The use of the (GTG)5 oligonucleotide, a repetitive marker in the Mycobacterium tuberculosis chromosome, as a primer in association with an IS6110 outlooking primer has been successfully applied to a PCR- based fingerprinting method. This method classified 62 strains of M. tuberculosis, isolated from human immunodeficiency virus-seropositive and -seronegative patients in different regions of Italy and Pakistan, as having 53 different patterns. The results were compared with traditional IS6110 fingerprinting, by which 47 distinct patterns were observed.


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