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Journal of Clinical Microbiology, Jul 1996, 1829-1830, Vol 34, No. 7
Copyright © 1996 by the American Society for Microbiology. All rights reserved.

Comparison of amplicor and 32-kilodalton PCR for detection of Mycobacterium tuberculosis from sputum specimens

H Soini, SA Agha, A El-Fiky and MK Viljanen
Department in Turku, National Public Health Institute, Finland.

The commercial PCR test Amplicor was compared with the 32-kDa PCR for detection of Mycobacterium tuberculosis from 76 sputum specimens from Egyptian patients. Both tests performed with rather equal efficacy (resolved sensitivity of 88.9% for both tests; specificity of 98.0% for Amplicor and 93.9% for 32-kDa PCR). PCR was found to be useful in detection of auramine fluorescent stain-positive, culture-negative specimens.


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