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Journal of Clinical Microbiology, Jan 1997, 264-267, Vol 35, No. 1
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

Typing of porcine reproductive and respiratory syndrome viruses by a multiplex PCR assay

SA Gilbert, R Larochelle, R Magar, HJ Cho and D Deregt
Animal Diseases Research Institute, Agriculture and Agri-Food Canada, Lethbridge, Alberta, Canada.

A rapid multiplex PCR assay was developed to distinguish between North American and European genotypes of porcine reproductive and respiratory syndrome (PRRS) virus after a portion of the polymerase gene (open reading frame 1b) was sequenced for two North American PRRS virus strains. DNA products with unique sizes characteristic of each genotype were obtained.

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