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Journal of Clinical Microbiology, 01 1997, 268-269, Vol 35, No. 1
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

Multiplex PCR for diagnosis of AIDS-related central nervous system lymphoma and toxoplasmosis

TC Roberts and GA Storch
Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110, USA.

A nested multiplex PCR assay was designed for the simultaneous detection of Epstein-Barr virus and Toxoplasma gondii DNA from the cerebrospinal fluid of AIDS patients. T. gondii DNA was detected in 8 of 8 patients with Toxoplasma encephalitis and in 0 of 6 patients without toxoplasmosis, and Epstein-Barr virus DNA was found in 9 of 14 patients with central nervous system lymphoma and in 2 of 38 patients without disease.


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