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Journal of Clinical Microbiology, Jan 1997, 270-272, Vol 35, No. 1
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

Lot-to-lot variability of antibiotic medium 3 used for testing susceptibility of Candida isolates to amphotericin B

M Lozano-Chiu, PW Nelson, M Lancaster, MA Pfaller and JH Rex
Department of Internal Medicine, University of Texas Medical School, Houston 77030, USA.

We have previously shown that use of antibiotic medium 3 in the microdilution variant of the M27-T test method permits detection of amphotericin B-resistant Candida isolates. As this medium is not standardized and our initial work used only a single lot of antibiotic medium 3, we studied the lot-to-lot variability of three commercial lots of antibiotic medium 3 obtained from two sources. The MICs obtained with the new lots were lower than those of with the original lot, but the new lots still consistently separated putatively resistant and putatively susceptible isolates and this permits proposal of possible breakpoints for this assay system.


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