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Journal of Clinical Microbiology, Jan 1997, 302-304, Vol 35, No. 1
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

Detection of herpes simplex virus in clinical specimens by cytospin- enhanced direct immunofluorescence

ML Landry, D Ferguson and J Wlochowski
Clinical Virology Laboratory, Yale New Haven Hospital, Connecticut, USA.

For 275 samples tested for herpes simplex virus (HSV), cytospin- enhanced direct immunofluorescence using Chemicon HSV monoclonal antibodies identified 80 (95%) and culture identified 77 (92%) of 84 confirmed positive specimens. Cytospin-prepared slides contained a greater number of total cells than standard cell spots, resulting in fewer inadequate cell smears and a higher HSV detection rate.


This article has been cited by other articles:

  • Landry, M. L., Cohen, S., Ferguson, D. (2008). Prospective Study of Human Metapneumovirus Detection in Clinical Samples by Use of Light Diagnostics Direct Immunofluorescence Reagent and Real-Time PCR. J. Clin. Microbiol. 46: 1098-1100 [Abstract] [Full Text]  
  • Leland, D. S., Ginocchio, C. C. (2007). Role of Cell Culture for Virus Detection in the Age of Technology. Clin. Microbiol. Rev. 20: 49-78 [Abstract] [Full Text]  
  • Chan, E. L., Brandt, K., Horsman, G. B. (2001). Comparison of Chemicon SimulFluor Direct Fluorescent Antibody Staining with Cell Culture and Shell Vial Direct Immunoperoxidase Staining for Detection of Herpes Simplex Virus and with Cytospin Direct Immunofluorescence Staining for Detection of Varicella-Zoster Virus. CVI 8: 909-912 [Abstract] [Full Text]  
  • Landry, M. L., Ferguson, D. (2000). SimulFluor Respiratory Screen for Rapid Detection of Multiple Respiratory Viruses in Clinical Specimens by Immunofluorescence Staining. J. Clin. Microbiol. 38: 708-711 [Abstract] [Full Text]  
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