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Journal of Clinical Microbiology, Oct 1997, 2464-2471, Vol 35, No. 10
MS Hughes, NW Ball, LA Beck, GW de Lisle, RA Skuce and SD Neill
PCR-amplified 16S rRNA gene sequences were obtained directly from tissue
specimens from eight cats with presumptive feline leprosy. Acid- fast
bacilli were observed in sections from all eight specimens, but culture for
mycobacteria was successful for one specimen only. Analysis of the V2
variable region of each 16S rRNA PCR product identified a sequence with
100% nucleotide identity to the sequences of Mycobacterium lepraemurium,
Mycobacterium avium, and Mycobacterium paratuberculosis in four of the
specimens from cats with feline leprosy. Separate M. paratuberculosis- and
M. avium-specific PCR amplifications of the four specimens were negative,
thus substantiating the identification of M. lepraemurium in these
specimens from cats with feline leprosy. Further sequence analysis of the
V3 variable region of one of the four specimens provided conclusive
evidence of the presence of M. lepraemurium. This is the first report of
the definitive identification of M. lepraemurium in cats with feline
leprosy by molecular biology-based analyses. M. avium, which is rarely
reported in cats, and Mycobacterium chitae, a reported nonpathogenic,
rapidly growing mycobacterial species found in the environment, were
identified in the specimen from which acid-fast bacilli were cultured. Two
of the specimens from cats were infected with a potentially novel species
of mycobacteria which had a 16S rRNA gene sequence sharing the closest
nucleotide sequence identity with that of Mycobacterium malmoense.
Molecular biology-based analyses provided for the accurate and rapid
diagnosis of mycobacterial infections in cats and circumvented the problems
of culture and misdiagnosis of feline leprosy associated with traditional
methods.
Copyright © 1997 by the American Society for Microbiology. All rights reserved.
Determination of the etiology of presumptive feline leprosy by 16S rRNA gene analysis
Department of Agriculture for Northern Ireland, Stormont, Belfast.
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