This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chatellier, S.
Right arrow Articles by Quentin, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chatellier, S.
Right arrow Articles by Quentin, R.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, Oct 1997, 2573-2579, Vol 35, No. 10
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

Characterization of Streptococcus agalactiae strains by randomly amplified polymorphic DNA analysis

S Chatellier, C Ramanantsoa, P Harriau, K Rolland, A Rosenau and R Quentin
Departement de Microbiologie Medicale et Moleculaire, Centre National de la Recherche Scientifique EP 117, Centre Hospitalier Universitaire Bretonneau, Tours, France.

A collection of 54 unrelated Streptococcus agalactiae strains isolated from cerebrospinal fluid samples from neonates and 60 unrelated strains isolated from carriers that had been previously studied by multilocus enzyme electrophoresis (R. Quentin, H. Huet, F.-S. Wang, P. Geslin, A. Goudeau, and R. K. Selander, J. Clin. Microbiol. 33:2576-2581, 1995) were characterized by randomly amplified polymorphic DNA (RAPD) assay. Four primers, 5'AGGGGGTTCC3', 5'AACGCGCAAC3', 5'GCATCAATCT3', and 5'AGTCGGGTGG3', named OPS16, AP42, A4, and OPS11, respectively, were selected from 29 primers tested. This investigation identified 71 RAPD types. The three families of strains defined by multilocus enzyme electrophoresis analysis, which contain most of the cerebrospinal fluid isolates, were also identified by clustering analysis of RAPD data. Each of these three groups exhibits specific RAPD patterns or fragments. The discriminatory power of the RAPD typing method was also evaluated. The simplest typing scheme was obtained by the combination of RAPD typing done with primers AP42 and OPS11 and serotyping (index of discrimination, 0.97).


This article has been cited by other articles:

  • Lobos, O., Padilla, C. (2009). Phenotypic characterization and genomic DNA polymorphisms of Escherichia coli strains isolated as the sole micro-organism from vaginal infections. Microbiology 155: 825-830 [Abstract] [Full Text]  
  • Puopolo, K. M., Klinzing, D. C., Lin, M. P., Yesucevitz, D. L., Cieslewicz, M. J. (2007). A composite transposon associated with erythromycin and clindamycin resistance in group B Streptococcus. J Med Microbiol 56: 947-955 [Abstract] [Full Text]  
  • Rosenau, A., Martins, K., Amor, S., Gannier, F., Lanotte, P., van der Mee-Marquet, N., Mereghetti, L., Quentin, R. (2007). Evaluation of the Ability of Streptococcus agalactiae Strains Isolated from Genital and Neonatal Specimens To Bind to Human Fibrinogen and Correlation with Characteristics of the fbsA and fbsB Genes. Infect. Immun. 75: 1310-1317 [Abstract] [Full Text]  
  • van der Mee-Marquet, N., Domelier, A.-S., Mereghetti, L., Lanotte, P., Rosenau, A., van Leeuwen, W., Quentin, R. (2006). Prophagic DNA Fragments in Streptococcus agalactiae Strains and Association with Neonatal Meningitis.. J. Clin. Microbiol. 44: 1049-1058 [Abstract] [Full Text]  
  • Mbwana, J., Bolin, I., Lyamuya, E., Mhalu, F., Lagergard, T. (2006). Molecular Characterization of Haemophilus ducreyi Isolates from Different Geographical Locations. J. Clin. Microbiol. 44: 132-137 [Abstract] [Full Text]  
  • Duarte, R. S., Bellei, B. C., Miranda, O. P., Brito, M. A. V. P., Teixeira, L. M. (2005). Distribution of Antimicrobial Resistance and Virulence-Related Genes among Brazilian Group B Streptococci Recovered from Bovine and Human Sources. Antimicrob. Agents Chemother. 49: 97-103 [Abstract] [Full Text]  
  • Watt, S., Lanotte, P., Mereghetti, L., Moulin-Schouleur, M., Picard, B., Quentin, R. (2003). Escherichia coli Strains from Pregnant Women and Neonates: Intraspecies Genetic Distribution and Prevalence of Virulence Factors. J. Clin. Microbiol. 41: 1929-1935 [Abstract] [Full Text]  
  • von Both, U., Ruess, M., Mueller, U., Fluegge, K., Sander, A., Berner, R. (2003). A Serotype V Clone Is Predominant among Erythromycin-Resistant Streptococcus agalactiae Isolates in a Southwestern Region of Germany. J. Clin. Microbiol. 41: 2166-2169 [Abstract] [Full Text]  
  • Rolland, K., Mereghetti, L., Watt, S., Chatellier, S., Quentin, R. (2002). tRNA gene clusters at the 3' end of rRNA operons are specific to virulent subgroups of Streptococcus agalactiae strains, as demonstrated by molecular differential analysis at the population level. Microbiology 148: 1493-1499 [Abstract] [Full Text]  
  • Martinez, G., Harel, J., Higgins, R., Lacouture, S., Daignault, D., Gottschalk, M. (2000). Characterization of Streptococcus agalactiae Isolates of Bovine and Human Origin by Randomly Amplified Polymorphic DNA Analysis. J. Clin. Microbiol. 38: 71-78 [Abstract] [Full Text]  
  • Rolland, K., Marois, C., Siquier, V., Cattier, B., Quentin, R. (1999). Genetic Features of Streptococcus agalactiae Strains Causing Severe Neonatal Infections, as Revealed by Pulsed-Field Gel Electrophoresis and hylB Gene Analysis. J. Clin. Microbiol. 37: 1892-1898 [Abstract] [Full Text]  
  • Arpin, C., Daube, H., Tessier, F., Quentin, C. (1999). Presence of mefA and mefE Genes in Streptococcus agalactiae. Antimicrob. Agents Chemother. 43: 944-946 [Abstract] [Full Text]  
  • Chatellier, S., Gottschalk, M., Higgins, R., Brousseau, R., Harel, J. (1999). Relatedness of Streptococcus suis Serotype 2 Isolates from Different Geographic Origins as Evaluated by Molecular Fingerprinting and Phenotyping. J. Clin. Microbiol. 37: 362-366 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»