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Journal of Clinical Microbiology, Oct 1997, 2665-2669, Vol 35, No. 10
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

Development of a method for direct quantification of cytomegalovirus antigenemia by flow cytometry

BM Imbert-Marcille, N Robillard, AS Poirier, M Coste-Burel, D Cantarovich, N Milpied and S Billaudel
Virology Laboratory, Institute of Biology of Nantes University Hospital, France. bmimbert@sante.univ-nantes.fr

Cytomegalovirus (CMV) antigenemia was directly detected in polymorphonuclear leukocytes (PMNLs) from transplant recipients by using flow cytometry (FC). Two fixation and permeabilization methods and seven anti-CMV monoclonal antibodies (MAbs) were evaluated. 1C3, SL20, and NEA-9221 MAbs were more efficacious. The antigenemia detection threshold of FC was 0.05% positive PMNLs, and percentages correlated well with DNA viral load and the appearance of clinical symptoms.


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