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Journal of Clinical Microbiology, Oct 1997, 2709-2711, Vol 35, No. 10
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

Immunomagnetic separation and PCR for detection of Mycobacterium ulcerans

B Roberts and R Hirst
Department of Microbiology and Immunology, James Cook University of North Queensland, Townsville, Australia. Brenda.Roberts@jcu.edu.au

We have developed a technique based on the use of monodisperse magnetic beads to isolate Mycobacterium ulcerans from heterogenous mixtures, prior to PCR amplification. Using this method, we were able to detect M. ulcerans in water samples taken from Phillip Island, Australia, the site of several outbreaks of M. ulcerans disease in recent times.


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