This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Teixeira, L. M.
Right arrow Articles by Facklam, R. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Teixeira, L. M.
Right arrow Articles by Facklam, R. R.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, 11 1997, 2778-2781, Vol 35, No. 11
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

Phenotypic and genotypic characterization of Vagococcus fluvialis, including strains isolated from human sources [In Process Citation]

LM Teixeira, MG Carvalho, VL Merquior, AG Steigerwalt, DJ Brenner and RR Facklam
Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Brazil. IMMMTML@microbio.ufrj.br

This study presents phenotypic and genotypic data for seven isolates of Vagococcus fluvialis, including four strains recovered from human clinical sources, one strain isolated from an environmental source, and two strains isolated from pigs. On the basis of phenotypic characteristics, most isolates were initially classified as "unidentified enterococci," because they resembled atypical arginine- negative enterococcal species. All seven strains as well as the type strain of V. fluvialis reacted with the AccuProbe Enterococcus genetic probe. The seven isolates had virtually indistinguishable whole-cell protein profiles that were similar to that of the V. fluvialis type strain and distinct from those of Enterococcus and Lactococcus species. DNA-DNA reassociation experiments confirmed that the strains were V. fluvialis. They were 71% or more related to the V. fluvialis type strain under optimum and stringent conditions, with 2.5% or less divergence within related sequences. All strains were susceptible to ampicillin, cefotaxime, trimethoprim-sulfamethoxazole, and vancomycin and were resistant to clindamycin, lomefloxacin, and ofloxacin. Strain- to-strain variation was observed in relation to susceptibilities to 18 other antimicrobial agents. Chromosomal DNA was analyzed by pulsed- field gel electrophoresis (PFGE) after digestion with SmaI. Distinctive PFGE patterns were generated, suggesting the nonclonal nature of V. fluvialis strains. Although the number of strains was small, this report provides molecular characterization of V. fluvialis and the first evidence of a possible connection of this species with human infections.


This article has been cited by other articles:

  • Bender, J. S., Kinyon, J. M., Kariyawasam, S., Halbur, P. G., Opriessnig, T. (2009). Comparison of conventional direct and enrichment culture methods for Erysipelothrix spp. from experimentally and naturally infected swine. jvdi 21: 863-868 [Abstract] [Full Text]  
  • Lawson, P. A., Falsen, E., Cotta, M. A., Whitehead, T. R. (2007). Vagococcus elongatus sp. nov., isolated from a swine-manure storage pit. Int. J. Syst. Evol. Microbiol. 57: 751-754 [Abstract] [Full Text]  
  • Duarte, R. S., Barros, R. R., Facklam, R. R., Teixeira, L. M. (2005). Phenotypic and Genotypic Characteristics of Streptococcus porcinus Isolated from Human Sources. J. Clin. Microbiol. 43: 4592-4601 [Abstract] [Full Text]  
  • Duarte, R. S., Bellei, B. C., Miranda, O. P., Brito, M. A. V. P., Teixeira, L. M. (2005). Distribution of Antimicrobial Resistance and Virulence-Related Genes among Brazilian Group B Streptococci Recovered from Bovine and Human Sources. Antimicrob. Agents Chemother. 49: 97-103 [Abstract] [Full Text]  
  • Shewmaker, P. L., Steigerwalt, A. G., Morey, R. E., Carvalho, M. d. G. S., Elliott, J. A., Joyce, K., Barrett, T. J., Teixeira, L. M., Facklam, R. R. (2004). Vagococcus carniphilus sp. nov., isolated from ground beef. Int. J. Syst. Evol. Microbiol. 54: 1505-1510 [Abstract] [Full Text]  
  • Duarte, R. S., Miranda, O. P., Bellei, B. C., Brito, M. A. V. P., Teixeira, L. M. (2004). Phenotypic and Molecular Characteristics of Streptococcus agalactiae Isolates Recovered from Milk of Dairy Cows in Brazil. J. Clin. Microbiol. 42: 4214-4222 [Abstract] [Full Text]  
  • Moore, J. E., Goldsmith, C. E., Elborn, J. S., Murphy, P. G., Gilligan, P. H., Fanning, S., Hogg, G. (2003). Towards "Molecular Esperanto" or the Tower of Babel? (The Need for Harmonization of Techniques for Genotyping Clinical Isolates of Pseudomonas aeruginosa Isolated from Patients with Cystic Fibrosis). J. Clin. Microbiol. 41: 5347-5348 [Full Text]  
  • Pellegrino, F. L. P. C., Teixeira, L. M., Carvalho, M. d. G. S., Aranha Nouer, S., Pinto de Oliveira, M., Mello Sampaio, J. L., D'Avila Freitas, A., Ferreira, A. L. P., Amorim, E. d. L. T., Riley, L. W., Moreira, B. M. (2002). Occurrence of a Multidrug-Resistant Pseudomonas aeruginosa Clone in Different Hospitals in Rio de Janeiro, Brazil. J. Clin. Microbiol. 40: 2420-2424 [Abstract] [Full Text]  
  • Teixeira, L. M., Barros, R. R., Castro, A. C. D., Peralta, J. M., Da Gloria S. Carvalho, M., Talkington, D. F., Vivoni, A. M., Facklam, R. R., Beall, B. (2001). Genetic and Phenotypic Features of Streptococcus pyogenes Strains Isolated in Brazil That Harbor New emm Sequences. J. Clin. Microbiol. 39: 3290-3295 [Abstract] [Full Text]  
  • Cappelli, E. A., Barros, R. R., Camello, T. C. F., Teixeira, L. M., Merquior, V. L. C. (1999). Leuconostoc pseudomesenteroides as a Cause of Nosocomial Urinary Tract Infections. J. Clin. Microbiol. 37: 4124-4126 [Abstract] [Full Text]  
  • Carvalho, M. D. G. S., Teixeira, L. M., Facklam, R. R. (1998). Use of Tests for Acidification of Methyl-alpha -D-Glucopyranoside and Susceptibility to Efrotomycin for Differentiation of Strains of Enterococcus and Some Related Genera. J. Clin. Microbiol. 36: 1584-1587 [Abstract] [Full Text]  
  • Fischer, W., Arneth-Seifert, D. (1998). D-Alanylcardiolipin, a Major Component of the Unique Lipid Pattern of Vagococcus fluvialis. J. Bacteriol. 180: 2950-2957 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»