This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Loffler, J.
Right arrow Articles by Einsele, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Loffler, J.
Right arrow Articles by Einsele, H.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, 12 1997, 3311-3312, Vol 35, No. 12
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

Comparison of different methods for extraction of DNA of fungal pathogens from cultures and blood [In Process Citation]

J Loffler, H Hebart, U Schumacher, H Reitze and H Einsele
Abt. Innere Medizin II, Medizinische Klinik und Poliklinik, Tubingen, Germany.

Five commercially available extraction kits and an in-house DNA extraction method for the release of DNA from Candida albicans and Aspergillus niger cells were assessed for sensitivity, purity, duration, and cost. All commercially available kits helped to shorten the duration of DNA extraction. However, the sensitivity varied from 1 to 1,000 fungal cells/ml and costs varied from $0.10 to 2.30. The QIAmp Tissue kit was the commercially available assay that yielded the same sensitivity and purity of fungal DNA release as the in-house protocol but was the most expensive method. In comparing these two extraction protocols, a 99% concordance of PCR results for 125 blood samples analyzed could be demonstrated.


This article has been cited by other articles:

  • Landlinger, C., Preuner, S., Willinger, B., Haberpursch, B., Racil, Z., Mayer, J., Lion, T. (2009). Species-Specific Identification of a Wide Range of Clinically Relevant Fungal Pathogens by Use of Luminex xMAP Technology. J. Clin. Microbiol. 47: 1063-1073 [Abstract] [Full Text]  
  • Metwally, L., Fairley, D. J., Coyle, P. V., Hay, R. J., Hedderwick, S., McCloskey, B., O'Neill, H. J., Webb, C. H., McMullan, R. (2008). Comparison of serum and whole-blood specimens for the detection of Candida DNA in critically ill, non-neutropenic patients. J Med Microbiol 57: 1269-1272 [Abstract] [Full Text]  
  • Francesconi, A., Kasai, M., Harrington, S. M., Beveridge, M. G., Petraitiene, R., Petraitis, V., Schaufele, R. L., Walsh, T. J. (2008). Automated and Manual Methods of DNA Extraction for Aspergillus fumigatus and Rhizopus oryzae Analyzed by Quantitative Real-Time PCR. J. Clin. Microbiol. 46: 1978-1984 [Abstract] [Full Text]  
  • Metwally, L., Fairley, D. J., Coyle, P. V., Hay, R. J., Hedderwick, S., McCloskey, B., O'Neill, H. J., Webb, C. H., Elbaz, W., McMullan, R. (2008). Improving molecular detection of Candida DNA in whole blood: comparison of seven fungal DNA extraction protocols using real-time PCR. J Med Microbiol 57: 296-303 [Abstract] [Full Text]  
  • Goldschmidt, P, Degorge, S, Saint-Jean, C, Year, H, Zekhnini, F, Batellier, L, Laroche, L, Chaumeil, C (2008). Resistance of Acanthamoeba to classic DNA extraction methods used for the diagnosis of corneal infections. Br J Ophthalmol 92: 112-115 [Abstract] [Full Text]  
  • Baskova, L., Landlinger, C., Preuner, S., Lion, T. (2007). The Pan-AC assay: a single-reaction real-time PCR test for quantitative detection of a broad range of Aspergillus and Candida species. J Med Microbiol 56: 1167-1173 [Abstract] [Full Text]  
  • Innings, A., Ullberg, M., Johansson, A., Rubin, C. J., Noreus, N., Isaksson, M., Herrmann, B. (2007). Multiplex Real-Time PCR Targeting the RNase P RNA Gene for Detection and Identification of Candida Species in Blood. J. Clin. Microbiol. 45: 874-880 [Abstract] [Full Text]  
  • Schabereiter-Gurtner, C., Selitsch, B., Rotter, M. L., Hirschl, A. M., Willinger, B. (2007). Development of Novel Real-Time PCR Assays for Detection and Differentiation of Eleven Medically Important Aspergillus and Candida Species in Clinical Specimens. J. Clin. Microbiol. 45: 906-914 [Abstract] [Full Text]  
  • Griffiths, L. J., Anyim, M., Doffman, S. R., Wilks, M., Millar, M. R., Agrawal, S. G. (2006). Comparison of DNA extraction methods for Aspergillus fumigatus using real-time PCR.. J Med Microbiol 55: 1187-1191 [Abstract] [Full Text]  
  • Procop, G. W. (2006). Evaluation of Molecular Diagnostic Assays for Fungal Infections. J. Mol. Diagn. 8: 297-298 [Full Text]  
  • White, P. L., Barton, R., Guiver, M., Linton, C. J., Wilson, S., Smith, M., Gomez, B. L., Carr, M. J., Kimmitt, P. T., Seaton, S., Rajakumar, K., Holyoake, T., Kibbler, C. C., Johnson, E., Hobson, R. P., Jones, B., Barnes, R. A., on behalf of the United Kingdom Fungal Polymerase, (2006). A Consensus on Fungal Polymerase Chain Reaction Diagnosis?: A United Kingdom-Ireland Evaluation of Polymerase Chain Reaction Methods for Detection of Systemic Fungal Infections. J. Mol. Diagn. 8: 376-384 [Abstract] [Full Text]  
  • Leinberger, D. M., Schumacher, U., Autenrieth, I. B., Bachmann, T. T. (2005). Development of a DNA Microarray for Detection and Identification of Fungal Pathogens Involved in Invasive Mycoses. J. Clin. Microbiol. 43: 4943-4953 [Abstract] [Full Text]  
  • Fredricks, D. N., Smith, C., Meier, A. (2005). Comparison of Six DNA Extraction Methods for Recovery of Fungal DNA as Assessed by Quantitative PCR. J. Clin. Microbiol. 43: 5122-5128 [Abstract] [Full Text]  
  • de Aguirre, L., Hurst, S. F., Choi, J. S., Shin, J. H., Hinrikson, H. P., Morrison, C. J. (2004). Rapid Differentiation of Aspergillus Species from Other Medically Important Opportunistic Molds and Yeasts by PCR-Enzyme Immunoassay. J. Clin. Microbiol. 42: 3495-3504 [Abstract] [Full Text]  
  • Maaroufi, Y., Ahariz, N., Husson, M., Crokaert, F. (2004). Comparison of Different Methods of Isolation of DNA of Commonly Encountered Candida Species and Its Quantitation by Using a Real-Time PCR-Based Assay. J. Clin. Microbiol. 42: 3159-3163 [Abstract] [Full Text]  
  • Warris, A., Klaassen, C. H. W., Meis, J. F. G. M., de Ruiter, M. T., de Valk, H. A., Abrahamsen, T. G., Gaustad, P., Verweij, P. E. (2003). Molecular Epidemiology of Aspergillus fumigatus Isolates Recovered from Water, Air, and Patients Shows Two Clusters of Genetically Distinct Strains. J. Clin. Microbiol. 41: 4101-4106 [Abstract] [Full Text]  
  • White, P. L., Shetty, A., Barnes, R. A. (2003). Detection of seven Candida species using the Light-Cycler system. J Med Microbiol 52: 229-238 [Abstract] [Full Text]  
  • Loeffler, J., Schmidt, K., Hebart, H., Schumacher, U., Einsele, H. (2002). Automated Extraction of Genomic DNA from Medically Important Yeast Species and Filamentous Fungi by Using the MagNA Pure LC System. J. Clin. Microbiol. 40: 2240-2243 [Abstract] [Full Text]  
  • Boddinghaus, B., Wichelhaus, T. A., Brade, V., Bittner, T. (2001). Removal of PCR Inhibitors by Silica Membranes: Evaluating the Amplicor Mycobacterium tuberculosis Kit. J. Clin. Microbiol. 39: 3750-3752 [Abstract] [Full Text]  
  • Ferrer, C., Colom, F., Frases, S., Mulet, E., Abad, J. L., Alio, J. L. (2001). Detection and Identification of Fungal Pathogens by PCR and by ITS2 and 5.8S Ribosomal DNA Typing in Ocular Infections. J. Clin. Microbiol. 39: 2873-2879 [Abstract] [Full Text]  
  • Lachaud, L., Chabbert, E., Dubessay, P., Reynes, J., Lamothe, J., Bastien, P. (2001). Comparison of Various Sample Preparation Methods for PCR Diagnosis of Visceral Leishmaniasis Using Peripheral Blood. J. Clin. Microbiol. 39: 613-617 [Abstract] [Full Text]  
  • Hammerschlag, M. R., Ke, Z., Lu, F., Roblin, P., Boman, J., Kalman, B. (2000). Is Chlamydia pneumoniae Present in Brain Lesions of Patients with Multiple Sclerosis?. J. Clin. Microbiol. 38: 4274-4276 [Abstract] [Full Text]  
  • Martin, C., Roberts, D., van der Weide, M., Rossau, R., Jannes, G., Smith, T., Maher, M. (2000). Development of a PCR-Based Line Probe Assay for Identification of Fungal Pathogens. J. Clin. Microbiol. 38: 3735-3742 [Abstract] [Full Text]  
  • Wahyuningsih, R., Freisleben, H.-J., Sonntag, H.-G., Schnitzler, P. (2000). Simple and Rapid Detection of Candida albicans DNA in Serum by PCR for Diagnosis of Invasive Candidiasis. J. Clin. Microbiol. 38: 3016-3021 [Abstract] [Full Text]  
  • Loeffler, J., Hagmeyer, L., Hebart, H., Henke, N., Schumacher, U., Einsele, H. (2000). Rapid Detection of Point Mutations by Fluorescence Resonance Energy Transfer and Probe Melting Curves in Candida Species. Clin. Chem. 46: 631-635 [Abstract] [Full Text]  
  • Becker, M. J., de Marie, S., Willemse, D., Verbrugh, H. A., Bakker-Woudenberg, I. A. J. M. (2000). Quantitative Galactomannan Detection Is Superior to PCR in Diagnosing and Monitoring Invasive Pulmonary Aspergillosis in an Experimental Rat Model. J. Clin. Microbiol. 38: 1434-1438 [Abstract] [Full Text]  
  • Reithinger, R., Lambson, B. E., Barker, D. C., Davies, C. R. (2000). Use of PCR To Detect Leishmania (Viannia) spp. in Dog Blood and Bone Marrow. J. Clin. Microbiol. 38: 748-751 [Abstract] [Full Text]  
  • Tran, S. D., Rudney, J. D. (1999). Improved Multiplex PCR Using Conserved and Species-Specific 16S rRNA Gene Primers for Simultaneous Detection of Actinobacillus actinomycetemcomitans, Bacteroides forsythus, and Porphyromonas gingivalis. J. Clin. Microbiol. 37: 3504-3508 [Abstract] [Full Text]  
  • Loeffler, J., Hebart, H., Bialek, R., Hagmeyer, L., Schmidt, D., Serey, F.-P., Hartmann, M., Eucker, J., Einsele, H. (1999). Contaminations Occurring in Fungal PCR Assays. J. Clin. Microbiol. 37: 1200-1202 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»