This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pfyffer, G. E.
Right arrow Articles by Rusch- Gerdes, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pfyffer, G. E.
Right arrow Articles by Rusch- Gerdes, S.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, Feb 1997, 364-368, Vol 35, No. 2
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

Comparison of the Mycobacteria Growth Indicator Tube (MGIT) with radiometric and solid culture for recovery of acid-fast bacilli

GE Pfyffer, HM Welscher, P Kissling, C Cieslak, MJ Casal, J Gutierrez and S Rusch- Gerdes
Department of Medical Microbiology, Swiss National Center for Mycobacteria, University of Zurich, Switzerland.

In a multicenter study involving three reference centers for mycobacteria, the rate of recovery of acid-fast bacilli (AFB) and the mean time to their detection from clinical specimens was determined by using the Mycobacteria Growth Indicator Tube (MGIT). These parameters were compared to those assessed by the radiometric BACTEC 460 TB system and by cultivation on solid media. Clinical specimens (n = 1,500) were pretreated with N-acetyl-L-cysteine (NALC)-NaOH. The contamination rates for MGITs were 2.0% (center 1), 13.8% (center 2), and 6.1% (center 3). A total of 180 mycobacterial isolates were detected (M. tuberculosis complex, n = 113; nontuberculous mycobacteria [NTM], n = 67). When using a combination of liquid and solid media (the current "gold standard" for culture), MGIT plus solid media detected 156 (86.7%) of the isolates, whereas BACTEC plus solid media recovered 168 (93.3%) of all AFB. Between these two gold standards there was no statistically significant difference (P > 0.05). The combination of MGIT plus BACTEC detected 171 (95.0%) of all isolates (compared with MGIT plus solid media, P < 0.01; compared with BACTEC plus solid media, P > 0.05). Considering the efficacies of the different media separately, MGIT was superior to solid media (although not significantly; P > 0.05) in detecting AFB but was inferior to the BACTEC system (P < 0.01). The mean time to the detection of M. tuberculosis complex was 9.9 days with MGIT, 9.7 days with BACTEC, and 20.2 days with solid media. NTM needed, on average, 11.9, 13.0, and 22.2 days to appear by the three methods, respectively. In conclusion, MGIT proved to be a valuable alternative to the radiometric cultivation system.


This article has been cited by other articles:

  • Greco, S., Rulli, M., Girardi, E., Piersimoni, C., Saltini, C. (2009). Diagnostic Accuracy of In-House PCR for Pulmonary Tuberculosis in Smear-Positive Patients: Meta-Analysis and Metaregression. J. Clin. Microbiol. 47: 569-576 [Abstract] [Full Text]  
  • Evans, J. T., Parveen, A., Smith, G. E., Xu, L., Chan, E. W. C., Chan, R. C. Y., Hawkey, P. M. (2009). Application of denaturing HPLC to rapidly identify rifampicin-resistant Mycobacterium tuberculosis in low- and high-prevalence areas. J Antimicrob Chemother 63: 295-301 [Abstract] [Full Text]  
  • Pardini, M., Varaine, F., Bonnet, M., Orefici, G., Oggioni, M. R., the LONG-DRUG Study Group, , Fattorini, L. (2007). Usefulness of the BACTEC MGIT 960 System for Isolation of Mycobacterium tuberculosis from Sputa Subjected to Long-Term Storage. J. Clin. Microbiol. 45: 575-576 [Abstract] [Full Text]  
  • Drobniewski, F. A., Hoffner, S., Rusch-Gerdes, S., Skenders, G., Thomsen, V., the WHO European Laboratory Strengthening Task For, (2006). Recommended standards for modern tuberculosis laboratory services in Europe. Eur Respir J 28: 903-909 [Abstract] [Full Text]  
  • Hillemann, D., Richter, E., Rusch-Gerdes, S. (2006). Use of the BACTEC Mycobacteria Growth Indicator Tube 960 Automated System for Recovery of Mycobacteria from 9,558 Extrapulmonary Specimens, Including Urine Samples. J. Clin. Microbiol. 44: 4014-4017 [Abstract] [Full Text]  
  • Greco, S, Girardi, E, Navarra, A, Saltini, C (2006). Current evidence on diagnostic accuracy of commercially based nucleic acid amplification tests for the diagnosis of pulmonary tuberculosis. Thorax 61: 783-790 [Abstract] [Full Text]  
  • Mathews, F., Macdonald, D. W, Taylor, G. M., Gelling, M., Norman, R. A, Honess, P. E, Foster, R., Gower, C. M, Varley, S., Harris, A., Palmer, S., Hewinson, G., Webster, J. P (2006). Bovine tuberculosis (Mycobacterium bovis) in British farmland wildlife: the importance to agriculture. Proc R Soc B 273: 357-365 [Abstract] [Full Text]  
  • Palomino, J. C. (2005). Nonconventional and new methods in the diagnosis of tuberculosis: feasibility and applicability in the field. Eur Respir J 26: 339-350 [Abstract] [Full Text]  
  • Rajalahti, I., Ruokonen, E-L., Kotomaki, T., Sintonen, H., Nieminen, M.M. (2004). Economic evaluation of the use of PCR assay in diagnosing pulmonary TB in a low-incidence area. Eur Respir J 23: 446-451 [Abstract] [Full Text]  
  • Migliori, G.B., Centis, R., Fattorini, L., Besozzi, G., Saltini, C., Orefici, G., Piersimoni, C., Gori, A., Cassone, A., the Italian Multicentre Study on Resistance to Ant, (2003). Monitoring the quality of laboratories and the prevalence of resistance to antituberculosis drugs: Italy, 1998-2000. Eur Respir J 21: 129-134 [Abstract] [Full Text]  
  • Miller, N., Cleary, T., Kraus, G., Young, A. K., Spruill, G., Hnatyszyn, H. J. (2002). Rapid and Specific Detection of Mycobacterium tuberculosis from Acid-Fast Bacillus Smear-Positive Respiratory Specimens and BacT/ALERT MP Culture Bottles by Using Fluorogenic Probes and Real-Time PCR. J. Clin. Microbiol. 40: 4143-4147 [Abstract] [Full Text]  
  • Chang, C. L., Park, T. S., Oh, S. H., Kim, H. H., Lee, E. Y., Son, H. C., Kim, C. M. (2002). Reduction of Contamination of Mycobacterial Growth Indicator Tubes with a Modified Antimicrobial Combination. J. Clin. Microbiol. 40: 3845-3847 [Abstract] [Full Text]  
  • Ruiz, P., Gutierrez, J., Zerolo, F. J., Casal, M. (2002). GenoType Mycobacterium Assay for Identification of Mycobacterial Species Isolated from Human Clinical Samples by Using Liquid Medium. J. Clin. Microbiol. 40: 3076-3078 [Abstract] [Full Text]  
  • Hasegawa, N., Miura, T., Ishii, K., Yamaguchi, K., Lindner, T. H., Merritt, S., Matthews, J. D., Siddiqi, S. H. (2002). New Simple and Rapid Test for Culture Confirmation of Mycobacterium tuberculosis Complex: a Multicenter Study. J. Clin. Microbiol. 40: 908-912 [Abstract] [Full Text]  
  • Zheng, X., Pang, M., Engler, H. D., Tanaka, S., Reppun, T. (2001). Rapid Detection of Mycobacterium tuberculosis in Contaminated BACTEC 12B Broth Cultures by Testing with Amplified Mycobacterium Tuberculosis Direct Test. J. Clin. Microbiol. 39: 3718-3720 [Abstract] [Full Text]  
  • Huang, T.-S., Chen, C.-S., Lee, S. S.-J., Huang, W.-K., Liu, Y.-C. (2001). Comparison of the BACTEC MGIT 960 and BACTEC 460TB Systems for Detection of Mycobacteria in Clinical Specimens. Annals of Clinical & Laboratory Science 31: 279-283 [Abstract] [Full Text]  
  • Hongmanee, P., Stender, H., Rasmussen, O. F. (2001). Evaluation of a Fluorescence In Situ Hybridization Assay for Differentiation between Tuberculous and Nontuberculous Mycobacterium Species in Smears of Lowenstein-Jensen and Mycobacteria Growth Indicator Tube Cultures Using Peptide Nucleic Acid Probes. J. Clin. Microbiol. 39: 1032-1035 [Abstract] [Full Text]  
  • Piersimoni, C., Scarparo, C., Callegaro, A., Tosi, C. P., Nista, D., Bornigia, S., Scagnelli, M., Rigon, A., Ruggiero, G., Goglio, A. (2001). Comparison of MB/BacT ALERT 3D System with Radiometric BACTEC System and Lowenstein-Jensen Medium for Recovery and Identification of Mycobacteria from Clinical Specimens: a Multicenter Study. J. Clin. Microbiol. 39: 651-657 [Abstract] [Full Text]  
  • Gascoyne-Binzi, D. M., Barlow, R. E. L., Frothingham, R., Robinson, G., Collyns, T. A., Gelletlie, R., Hawkey, P. M. (2001). Rapid Identification of Laboratory Contamination with Mycobacterium tuberculosis Using Variable Number Tandem Repeat Analysis. J. Clin. Microbiol. 39: 69-74 [Abstract] [Full Text]  
  • Watterson, S A, Drobniewski, F A (2000). Modern laboratory diagnosis of mycobacterial infections. J. Clin. Pathol. 53: 727-732 [Abstract] [Full Text]  
  • Somoskövi, A., Ködmön, C., Lantos, A., Bártfai, Z., Tamási, L., Füzy, J., Magyar, P. (2000). Comparison of Recoveries of Mycobacterium tuberculosis Using the Automated BACTEC MGIT 960 System, the BACTEC 460 TB System, and Lowenstein-Jensen Medium. J. Clin. Microbiol. 38: 2395-2397 [Abstract] [Full Text]  
  • Miller, N., Infante, S., Cleary, T. (2000). Evaluation of the LiPA MYCOBACTERIA Assay for Identification of Mycobacterial Species from BACTEC 12B Bottles. J. Clin. Microbiol. 38: 1915-1919 [Abstract] [Full Text]  
  • Katila, M.-L., Katila, P., Erkinjuntti-Pekkanen, R. (2000). Accelerated Detection and Identification of Mycobacteria with MGIT 960 and COBAS AMPLICOR Systems. J. Clin. Microbiol. 38: 960-964 [Abstract] [Full Text]  
  • Heifets, L., Linder, T., Sanchez, T., Spencer, D., Brennan, J. (2000). Two Liquid Medium Systems, Mycobacteria Growth Indicator Tube and MB Redox Tube, for Mycobacterium tuberculosis Isolation from Sputum Specimens. J. Clin. Microbiol. 38: 1227-1230 [Abstract] [Full Text]  
  • Alcaide, F., Benítez, M. A., Escribà, J. M., Martín, R. (2000). Evaluation of the BACTEC MGIT 960 and the MB/BacT Systems for Recovery of Mycobacteria from Clinical Specimens and for Species Identification by DNA AccuProbe. J. Clin. Microbiol. 38: 398-401 [Abstract] [Full Text]  
  • Tortoli, E., Cichero, P., Piersimoni, C., Simonetti, M. T., Gesu, G., Nista, D. (1999). Use of BACTEC MGIT 960 for Recovery of Mycobacteria from Clinical Specimens: Multicenter Study. J. Clin. Microbiol. 37: 3578-3582 [Abstract] [Full Text]  
  • Abe, C., Hirano, K., Tomiyama, T. (1999). Simple and Rapid Identification of the Mycobacterium tuberculosis Complex by Immunochromatographic Assay Using Anti-MPB64 Monoclonal Antibodies. J. Clin. Microbiol. 37: 3693-3697 [Abstract] [Full Text]  
  • Roggenkamp, A., Hornef, M. W., Masch, A., Aigner, B., Autenrieth, I. B., Heesemann, J. (1999). Comparison of MB/BacT and BACTEC 460 TB Systems for Recovery of Mycobacteria in a Routine Diagnostic Laboratory. J. Clin. Microbiol. 37: 3711-3712 [Abstract] [Full Text]  
  • Pfyffer, G. E., Bonato, D. A., Ebrahimzadeh, A., Gross, W., Hotaling, J., Kornblum, J., Laszlo, A., Roberts, G., Salfinger, M., Wittwer, F., Siddiqi, S. (1999). Multicenter Laboratory Validation of Susceptibility Testing of Mycobacterium tuberculosis against Classical Second-Line and Newer Antimicrobial Drugs by Using the Radiometric BACTEC 460 Technique and the Proportion Method with Solid Media. J. Clin. Microbiol. 37: 3179-3186 [Abstract] [Full Text]  
  • Cambau, E., Wichlacz, C., Truffot-Pernot, C., Jarlier, V. (1999). Evaluation of the New MB Redox System for Detection of Growth of Mycobacteria. J. Clin. Microbiol. 37: 2013-2015 [Abstract] [Full Text]  
  • Somoskövi, A., Magyar, P. (1999). Comparison of the Mycobacteria Growth Indicator Tube with MB Redox, Lowenstein-Jensen, and Middlebrook 7H11 Media for Recovery of Mycobacteria in Clinical Specimens. J. Clin. Microbiol. 37: 1366-1369 [Abstract] [Full Text]  
  • Brunello, F., Favari, F., Fontana, R. (1999). Comparison of the MB/BacT and BACTEC 460 TB Systems for Recovery of Mycobacteria from Various Clinical Specimens. J. Clin. Microbiol. 37: 1206-1209 [Abstract] [Full Text]  
  • Rüsch-Gerdes, S., Domehl, C., Nardi, G., Gismondo, M. R., Welscher, H.-M., Pfyffer, G. E. (1999). Multicenter Evaluation of the Mycobacteria Growth Indicator Tube for Testing Susceptibility of Mycobacterium tuberculosis to First-Line Drugs. J. Clin. Microbiol. 37: 45-48 [Abstract] [Full Text]  
  • Tortoli, E., Lavinia, F., Simonetti, M. T. (1998). Early Detection of Mycobacterium tuberculosis in BACTEC Cultures by Ligase Chain Reaction. J. Clin. Microbiol. 36: 2791-2792 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»