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Journal of Clinical Microbiology, Feb 1997, 492-494, Vol 35, No. 2
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

Mutations in the rpoB gene of Mycobacterium tuberculosis that interfere with PCR-single-strand conformation polymorphism analysis for rifampin susceptibility testing

BJ Kim, SY Kim, BH Park, MA Lyu, IK Park, GH Bai, SJ Kim, CY Cha and YH Kook
Department of Microbiology and Cancer Research Center, Seoul National University College of Medicine, Korea.

Rifampin susceptibility of 32 rifampin-resistant and 26 rifampin- susceptible Mycobacterium tuberculosis strains was analyzed by PCR- single-strand conformation polymorphism (SSCP) and DNA sequencing within the 157-bp region of the rpoB gene (Ala500 to Val550). Two false- positive PCR-SSCP results were observed among the susceptible strains due to the silent mutation Gln513 (CAA-->CAG) and the deletion mutation Thr508 and Ser509. Another silent mutation [Leu511 (CTG-->CTA)], combined with the mutation Ser531-->Leu, was observed in a resistant strain. These results suggest that to rule out false-positive PCR-SSCP results, sequencing of the target DNA is required.


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