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Journal of Clinical Microbiology, Mar 1997, 741-744, Vol 35, No. 3
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

Minimal effect of delayed sample processing on results of quantitative PCR for cytomegalovirus DNA in leukocytes compared to results of an antigenemia assay

P Schafer, W Tenschert, K Gutensohn and R Laufs
Institut fur Medizinische Mikrobiologie und Immunologie, Universitats- Krankenhaus Eppendorf, Hamburg, Germany.

Quantitative cytomegalovirus antigenemia and DNAemia were determined in peripheral leukocytes of 25 patients stored for up to 72 h at room temperature (RT) and 4 degrees C before processing. Numbers of antigen- positive cells significantly decreased with time. The decline was greater at RT than at 4 degrees C. In contrast, no significant alterations in DNAemia occurred.


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