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Journal of Clinical Microbiology, Jun 1997, 1620-1623, Vol 35, No. 6
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

Fecal PCR assay for diagnosis of Helicobacter infection in laboratory rodents

CS Beckwith, CL Franklin, RR Hook Jr, CL Besch-Williford and LK Riley
Department of Veterinary Pathobiology, University of Missouri, Columbia 65211, USA. vmkatie@vetmed.missouri.edu

A fecal PCR assay for detection of Helicobacter infections in laboratory rodents was developed. DNA was isolated from murine fecal pellets, and a region of the 16S rRNA gene conserved among murine Helicobacter species was amplified. The fecal PCR was sensitive and specific. This assay does not require euthanasia of rodents, which is especially important for valuable rodents, such as transgenic mice.


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