This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Valdez, L. M.
Right arrow Articles by Chappell, C. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Valdez, L. M.
Right arrow Articles by Chappell, C. L.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, 08 1997, 2013-2017, Vol 35, No. 8
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

Flow cytometric detection of Cryptosporidium oocysts in human stool samples

LM Valdez, H Dang, PC Okhuysen and CL Chappell
Center for Infectious Diseases, School of Public Health, The University of Texas Houston Health Science Center, 77030, USA.

Cryptosporidium parvum is an important pathogen that causes diarrhea in virtually all human populations. Improved diagnostic methods are needed to understand the risk factors, modes of transmission, and impact of cryptosporidiosis. In the present study, we fluorescently labeled and counted C. parvum oocysts by flow cytometry (FC) and developed a simple and efficient method of processing human stool samples for FC analysis. Formed stool (suspended in phosphate-buffered saline) from an asymptomatic, healthy individual was seeded with known concentrations of oocysts, and oocysts were labeled with a cell wall-specific monoclonal antibody and detected by FC. The method described herein resulted in a mean oocyst recovery rate of 45% +/- 16% (median, 42%), which consistently yielded a fourfold increase in sensitivity compared to direct fluorescent-antibody assay of seeded stool samples. However, in many instances, FC detected as few as 10(3) oocysts per ml. Thus, FC provides a reproducible and sensitive method for C. parvum oocyst detection.


This article has been cited by other articles:

  • Uehlinger, F. D., Barkema, H. W., O'Handley, R. M., Parenteau, M., Parrington, L. J., VanLeeuwen, J. A., Dixon, B. R. (2008). Comparison of flow cytometry and immunofluorescence microscopy for the detection of Giardia duodenalis in bovine fecal samples. jvdi 20: 178-185 [Abstract] [Full Text]  
  • Gargala, G., Baishanbo, A., Favennec, L., Francois, A., Ballet, J. J., Rossignol, J.-F. (2005). Inhibitory Activities of Epidermal Growth Factor Receptor Tyrosine Kinase-Targeted Dihydroxyisoflavone and Trihydroxydeoxybenzoin Derivatives on Sarcocystis neurona, Neospora caninum, and Cryptosporidium parvum Development. Antimicrob. Agents Chemother. 49: 4628-4634 [Abstract] [Full Text]  
  • Dixon, B. R., Bussey, J. M., Parrington, L. J., Parenteau, M. (2005). Detection of Cyclospora cayetanensis Oocysts in Human Fecal Specimens by Flow Cytometry. J. Clin. Microbiol. 43: 2375-2379 [Abstract] [Full Text]  
  • Tanriverdi, S., Tanyeli, A., Baslamisli, F., Koksal, F., Kilinc, Y., Feng, X., Batzer, G., Tzipori, S., Widmer, G. (2002). Detection and Genotyping of Oocysts of Cryptosporidium parvum by Real-Time PCR and Melting Curve Analysis. J. Clin. Microbiol. 40: 3237-3244 [Abstract] [Full Text]  
  • Cole, D. J., Snowden, K., Cohen, N. D., Smith, R. (1999). Detection of Cryptosporidium parvum in Horses: Thresholds of Acid-Fast Stain, Immunofluorescence Assay, and Flow Cytometry. J. Clin. Microbiol. 37: 457-460 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»