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Journal of Clinical Microbiology, Aug 1997, 2142-2144, Vol 35, No. 8
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

Pretreatment of clinical specimens with sodium dodecyl (lauryl) sulfate is not suitable for the mycobacteria growth indicator tube cultivation method

GE Pfyffer, HM Welscher and P Kissling
Swiss National Center for Mycobacteria, Department of Medical Microbiology, University of Zurich.

When using the Mycobacteria Growth Indicator Tube (MGIT), pretreatment of clinical specimens with N-acetyl-L-cysteine-NaOH is recommended by the manufacturer. Processing of clinical specimens (n = 1,000) with sodium dodecyl (lauryl) sulfate-NaOH resulted in both poor recovery and delayed mean time to detection of acid-fast bacilli. Values were comparable to those obtained on solid media.




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