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Journal of Clinical Microbiology, Aug 1997, 2155-2156, Vol 35, No. 8
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

Simple color tests based on an alanyl peptidase reaction which differentiate Listeria monocytogenes from other Listeria species

AG Clark and J McLaughlin
Department of Medical Genetics and Microbiology, University of Toronto, Ontario, Canada. gavin.clark@utoronto.ca

The hydrolysis of DL-alanine-beta-naphthylamide and D-alanine-p- nitroanilide for identification of Listeria spp. has been studied with 227 cultures. All species of Listeria, except L. monocytogenes, hydrolyzed these substrates. The reactions were detected by simple chromogenic reactions and could substitute for the CAMP test.

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