This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wilson, R. W.
Right arrow Articles by Wallace, R. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wilson, R. W.
Right arrow Articles by Wallace, R. J., Jr

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, 09 1997, 2235-2242, Vol 35, No. 9
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

Recognition of a Nocardia transvalensis complex by resistance to aminoglycosides, including amikacin, and PCR-restriction fragment length polymorphism analysis

RW Wilson, VA Steingrube, BA Brown, Z Blacklock, KC Jost Jr, A McNabb, WD Colby, JR Biehle, JL Gibson and RJ Wallace Jr
Center for Pulmonary and Infectious Disease Control, The University of Texas Health Center at Tyler, 75710-2003, USA.

Amikacin resistance, rare among nocardiae, was observed in 58 clinical isolates of nocardiae. All of these isolates hydrolyzed hypoxanthine, and 75 to 100% utilized citrate, D-galactose, and D-trehalose as sole carbon sources. Based on utilization of I-erythritol, D-glucitol, i-myo- inositol, D-mannitol, and ribitol and susceptibility to amoxicillin- clavulanic acid, the 58 isolates were separable into four groups. One group was negative for I-erythritol and ribitol and included all the isolates belonging to Nocardia asteroides complex antibiogram type IV. The remaining three groups were positive for I-erythritol and ribitol and were grouped within Nocardia transvalensis. The group that included the type strain was designated N. transvalensis sensu stricto, and the other two groups were designated new taxons 1 and 2. PCR-restriction fragment length polymorphism (RFLP) analysis of a 439-bp segment of the 65-kDa heat shock protein gene with XhoI and HinfI produced identical patterns for 53 (91%) and 58 (100%) isolates, respectively, and differentiated them from all other Nocardia taxa. NarI- and HaeIII- derived RFLP patterns clearly differentiated each of the four biochemically defined taxa. These four groups were also distinguishable by using the chromogenic substrates in Dade MicroScan test panels. By high-performance liquid chromatography, these isolates exhibited the same unique mycolic acid-ester elution patterns that differed from those of all other clinically significant nocardiae. Gas-liquid chromatographic analysis of fatty acids also produced similar patterns for all isolates that distinguished them from all other Nocardia taxa, but did not differentiate the four taxa within the complex. We propose the designation N. transvalensis complex for these four groups of nocardiae, pending further genetic evaluation.


This article has been cited by other articles:

  • Conville, P. S., Brown, J. M., Steigerwalt, A. G., Brown-Elliott, B. A., Witebsky, F. G. (2008). Nocardia wallacei sp. nov. and Nocardia blacklockiae sp. nov., Human Pathogens and Members of the "Nocardia transvalensis Complex". J. Clin. Microbiol. 46: 1178-1184 [Abstract] [Full Text]  
  • Ramos-Vara, J. A., Wu, C. C., Lin, T. L., Miller, M. A. (2007). Nocardia tenerifensis genome identification in a cutaneous granuloma of a cat. jvdi 19: 577-580 [Abstract] [Full Text]  
  • Brown-Elliott, B. A., Brown, J. M., Conville, P. S., Wallace, R. J. Jr (2006). Clinical and Laboratory Features of the Nocardia spp. Based on Current Molecular Taxonomy. Clin. Microbiol. Rev. 19: 259-282 [Abstract] [Full Text]  
  • Patel, J. B., Wallace, R. J. Jr., Brown-Elliott, B. A., Taylor, T., Imperatrice, C., Leonard, D. G. B., Wilson, R. W., Mann, L., Jost, K. C., Nachamkin, I. (2004). Sequence-Based Identification of Aerobic Actinomycetes. J. Clin. Microbiol. 42: 2530-2540 [Abstract] [Full Text]  
  • Gomez-Flores, A., Welsh, O., Said-Fernandez, S., Lozano-Garza, G., Tavarez-Alejandro, R. E., Vera-Cabrera, L. (2004). In Vitro and In Vivo Activities of Antimicrobials against Nocardia brasiliensis. Antimicrob. Agents Chemother. 48: 832-837 [Abstract] [Full Text]  
  • Cloud, J. L., Conville, P. S., Croft, A., Harmsen, D., Witebsky, F. G., Carroll, K. C. (2004). Evaluation of Partial 16S Ribosomal DNA Sequencing for Identification of Nocardia Species by Using the MicroSeq 500 System with an Expanded Database. J. Clin. Microbiol. 42: 578-584 [Abstract] [Full Text]  
  • Godreuil, S., Didelot, M.-N., Perez, C., Lefleche, A., Boiron, P., Reynes, J., Laurent, F., Jean-Pierre, H., Marchandin, H. (2003). Nocardia veterana Isolated from Ascitic Fluid of a Patient with Human Immunodeficiency Virus Infection. J. Clin. Microbiol. 41: 2768-2773 [Abstract] [Full Text]  
  • Roth, A., Andrees, S., Kroppenstedt, R. M., Harmsen, D., Mauch, H. (2003). Phylogeny of the Genus Nocardia Based on Reassessed 16S rRNA Gene Sequences Reveals Underspeciation and Division of Strains Classified as Nocardia asteroides into Three Established Species and Two Unnamed Taxons. J. Clin. Microbiol. 41: 851-856 [Abstract] [Full Text]  
  • Kiska, D. L., Hicks, K., Pettit, D. J. (2002). Identification of Medically Relevant Nocardia Species with an Abbreviated Battery of Tests. J. Clin. Microbiol. 40: 1346-1351 [Abstract] [Full Text]  
  • Brown-Elliott, B. A., Ward, S. C., Crist, C. J., Mann, L. B., Wilson, R. W., Wallace, R. J. Jr. (2001). In Vitro Activities of Linezolid against Multiple Nocardia Species. Antimicrob. Agents Chemother. 45: 1295-1297 [Abstract] [Full Text]  
  • Conville, P. S., Fischer, S. H., Cartwright, C. P., Witebsky, F. G. (2000). Identification of Nocardia Species by Restriction Endonuclease Analysis of an Amplified Portion of the 16S rRNA Gene. J. Clin. Microbiol. 38: 158-164 [Abstract] [Full Text]  
  • Wilson, R., Steingrube, V., Brown, B., Wallace, R. Jr (1998). Clinical application of PCR-restriction enzyme pattern analysis for rapid identification of aerobic actinomycete isolates [In Process Citation]. J. Clin. Microbiol. 36: 148-152 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»