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Journal of Clinical Microbiology, Sep 1997, 2405-2407, Vol 35, No. 9
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

Comparison of use of enzyme-linked immunosorbent assay-based kits and PCR amplification of rRNA genes for simultaneous detection of Entamoeba histolytica and E. dispar

D Mirelman, Y Nuchamowitz and T Stolarsky
Department of Membrane Research and Biophysics, Weizmann Institute of Science, Rehovot, Israel. bfmirelm@weizmann.weizmann.ac.il

A comparison of the use of three commercially available enzyme-linked immunosorbent assay-based kits and PCR amplification of rRNA genes to detect and differentiate Entamoeba histolytica from E. dispar was carried out. Only the Techlab kit did not cross-react with E. dispar antigens, but it was 100 times less sensitive than PCR in detection of and differentiation between the two types of Entamoeba.


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