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Journal of Clinical Microbiology, 09 1997, 2419-2421, Vol 35, No. 9
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

Subtyping of human immunodeficiency virus type 1 strains by using antibodies specific for the third variable domain (V3) of gp120: results may be affected by divergent V3 sequences

K Sherefa, M Sallberg, B Johansson, M Salminen and A Sonnerborg
Department of Immunology, Microbiology, Pathology, and Infectious Diseases, Karolinska Institutet, Huddinge Hospital, Sweden.

Human immunodeficiency virus type 1 serotype C was found in 545 of 712 Ethiopian patients by peptide enzyme immunoassay. Serotyping failed in 146 samples due to the absence of V3 antibodies or multiple reactivities. In 6 of 34 samples, discordant results were obtained by serotyping and genotyping, possibly due to divergent V3 sequences.


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