This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Limoncu, M. E. Articles by Ozbilgin, A. Search for Related Content PubMed PubMed Citation Articles by Limoncu, M. E. Articles by Ozbilgin, A.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, 09 1997, 2430-2431, Vol 35, No. 9
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

A new experimental in vitro culture medium for cultivation of Leishmania species

ME Limoncu, IC Balcioglu, K Yereli, Y Ozbel and A Ozbilgin
Department of Microbiology, Celal Bayar University School of Medicine, Manisa, Turkey.

A new liquid culture medium prepared with chemicals that can be obtained economically and commercially was tested in in vitro cultivation of Leishmania promastigotes to obtain a large number of organisms to use in serological studies. The number of Leishmania infantum and Leishmania tropica promastigotes taken from Novy-MacNeal- Nicolle (NNN) medium reached 1 x 10(7)/ml at the end of the 8th day in our new medium, though in NNN medium the number of organisms reached only 5 x 10(6)/ml. After 10 subsequent passages, the culture medium prepared was evaluated as being quite inexpensive, simple, and successful compared with other commercially available liquid culture media.

This article has been cited by other articles:

• Schuster, F. L., Sullivan, J. J. (2002). Cultivation of Clinically Significant Hemoflagellates. Clin. Microbiol. Rev. 15: 374-389 [Abstract] [Full Text]