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Journal of Clinical Microbiology, January 1998, p. 1-5, Vol. 36, No. 1
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

First Isolation and Cultivation of Borrelia burgdorferi Sensu Lato from Missouri

J. H. Oliver Jr.,1,* T. M. Kollars Jr.,1 F. W. Chandler Jr.,2 A. M. James,1,dagger E. J. Masters,3 R. S. Lane,4 and L. O. Huey2

Institute of Arthropodology and Parasitology, Georgia Southern University, Statesboro, Georgia 30460-80561; Department of Pathology, BF-230, Medical College of Georgia, Augusta, Georgia 30912-36052; Family Physicians Group, Inc., Cape Girardeau, Missouri 63701-49803; and Department of Entomological Science and Parasitology, University of California at Berkeley, Berkeley, California 947204

Received 20 June 1997/Returned for modification 31 July 1997/Accepted 24 September 1997

Five Borrelia burgdorferi sensu lato isolates from Missouri are described. This represents the first report and characterization of such isolates from that state. The isolates were obtained from either Ixodes dentatus or Amblyomma americanum ticks that had been feeding on cottontail rabbits (Sylvilagus floridanus) from a farm in Bollinger County, Mo., where a human case of Lyme disease had been reported. All isolates were screened immunologically by indirect immunofluorescence by using monoclonal antibodies to B. burgdorferi-specific outer surface protein A (OspA) (antibodies H3TS and H5332), B. burgdorferi-specific OspB (antibody H6831), Borrelia (genus)-specific antiflagellin (antibody H9724), and Borrelia hermsii-specific antibody (antibody H9826). Analysis of the isolates also involved a comparison of their protein profiles by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Finally, the isolates were analyzed by PCR with six pairs of primers known to amplify selected DNA target sequences specifically found in the reference strain B. burgdorferi B-31. Although some genetic variability was detected among the five isolates as well as between them and the B-31 strain, enough similarities were found to classify them as B. burgdorferi sensu lato.

* Corresponding author. Mailing address: Institute of Arthropodology and Parasitology, Georgia Southern University, P.O. Box 8056, Statesboro, GA 30460-8056. Phone: (912) 681-5564. Fax: (912) 681-0559. E-mail: JOliver{at}gasou.edu.

dagger Present address: Division of Vector-Borne Infectious Disease, Centers for Disease Control and Prevention, Fort Collins, CO 80522.

Journal of Clinical Microbiology, January 1998, p. 1-5, Vol. 36, No. 1
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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